Difference between revisions of "Team:NYU Shanghai/Protocols"
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<br><br>Materials | <br><br>Materials | ||
<li>2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes) | <li>2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes) | ||
− | <li>Competent cells (50ul per transformation) | + | <li>Competent cells (50ul per transformation) |
− | <li>Ice (in ice bucket/container) | + | <li>Ice (in ice bucket/container) |
− | <li>2ml tube (1 per a transformation') | + | <li>2ml tube (1 per a transformation') |
− | <li>42ºC water bath | + | <li>42ºC water bath |
− | <li>SOC media | + | <li>SOC media |
− | <li>1 small LB plate and 1 large LB+antibiotic per transformation | + | <li>1 small LB plate and 1 large LB+antibiotic per transformation |
− | <li>Spreader | + | <li>Spreader |
− | <li>37ºC incubator | + | <li>37ºC incubator |
<br>Procedure | <br>Procedure | ||
<ol> | <ol> |
Revision as of 19:16, 11 September 2015