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Revision as of 12:43, 13 September 2015
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We built our own simplified and affordable detection device. Check out the step by step instructions!
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We provided a new backbone for protein overexpression meeting all the iGEM standards.
See how we epanded the iGEM registry! -
What do people think about the DiaCHIP and systems based on synthetic biology?
Check out the results of our survey and discussions! -
Modeling the generation of proteins during cell-free expression as well as their diffusion in our system helped us obtaining better results. Get more information here.
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We successfully detected tetanus antibodies in human blood serum and measured binding of GFP antibodies to cell-free expressed GFP.
Visit our results page! -
Nowadays, medical diagnosis is mainly based on huge amounts of time-consuming and costly serological tests. Read more about the issues we tackled!
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Simultaneously screening for hundreds of diseases within a few hours?
Find out about the DiaCHIP's potential to revolutionize future diagnostics! -
Generating a protein array on demand via cell-free expression and detecting antigen-antibody interactions label-free and in real-time: The principle behind the DiaCHIP!
Modern everyday life is fast...
...too fast for spending unnecessary time at the doctor’s and waiting weeks for your test results.
Not to mention spending lots of money on numerous serological tests for your diagnosis. Wouldn't life be easier if a single test was sufficient?
This is the reason why we thought about a universally accessible, cost and time efficient diagnostic device and developped the DiaCHIP!
DiaCHIP - Enlightening Diagnostics
In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference). Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.
