Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"
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| − | <p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:</p> | + | <p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following unique BioBrick combinations:</p> |
<div align=center> | <div align=center> | ||
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<tr> | <tr> | ||
| + | <td><b><u>Combination #</u></b></td> | ||
<td><b><u>Fluorescent Protein BioBrick</u></b></td> | <td><b><u>Fluorescent Protein BioBrick</u></b></td> | ||
<td><b><u>Fluorescent Protein</u></b></td> | <td><b><u>Fluorescent Protein</u></b></td> | ||
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<tr></tr> | <tr></tr> | ||
<tr> | <tr> | ||
| + | <td>1</td> | ||
<td>BBa_K592100</td> | <td>BBa_K592100</td> | ||
<td>BFP</td> | <td>BFP</td> | ||
| Line 30: | Line 32: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>2</td> | ||
<td>BBa_K592100</td> | <td>BBa_K592100</td> | ||
<td>BFP</td> | <td>BFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>3</td> | ||
<td>BBa_E0020</td> | <td>BBa_E0020</td> | ||
<td>ECFP</td> | <td>ECFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>4</td> | ||
<td>BBa_E0020</td> | <td>BBa_E0020</td> | ||
<td>ECFP</td> | <td>ECFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>5</td> | ||
<td>BBa_E0020</td> | <td>BBa_E0020</td> | ||
<td>ECFP</td> | <td>ECFP</td> | ||
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<hr> | <hr> | ||
<tr> | <tr> | ||
| + | <td>6</td> | ||
<td>BBa_K592101</td> | <td>BBa_K592101</td> | ||
<td>YFP</td> | <td>YFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>7</td> | ||
<td>BBa_K592101</td> | <td>BBa_K592101</td> | ||
<td>YFP</td> | <td>YFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>8</td> | ||
<td>BBa_K592101</td> | <td>BBa_K592101</td> | ||
<td>YFP</td> | <td>YFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>9</td> | ||
<td>BBa_K864100</td> | <td>BBa_K864100</td> | ||
<td>SYFP2</td> | <td>SYFP2</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>10</td> | ||
<td>BBa_K864100</td> | <td>BBa_K864100</td> | ||
<td>SYFP2</td> | <td>SYFP2</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>11</td> | ||
<td>BBa_K864100</td> | <td>BBa_K864100</td> | ||
<td>SYFP2</td> | <td>SYFP2</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>12</td> | ||
<td>BBa_E0030</td> | <td>BBa_E0030</td> | ||
<td>EYFP</td> | <td>EYFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>13</td> | ||
<td>BBa_EE030</td> | <td>BBa_EE030</td> | ||
<td>EYFP</td> | <td>EYFP</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| + | <td>14</td> | ||
<td>BBa_E0030</td> | <td>BBa_E0030</td> | ||
<td>EYFP</td> | <td>EYFP</td> | ||
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<div align=left> | <div align=left> | ||
<br> | <br> | ||
| − | <font color="017e70">plasmids transformed by electroporation into pSB1c3</font> | + | <font color="017e70">plasmids transformed by electroporation into pSB1c3</font><br> |
| + | Several fluorescent protein/promoter+RBS combinations were studied in more than one experiment, which is why each is classified as a 'unique' combination.</br> | ||
| + | <br> | ||
| + | Each BioBrick combination was | ||
<br> | <br> | ||
Revision as of 22:56, 13 September 2015
--this is a first draft--
Spring 2015 Data Set
Plasmid Construction and Transformation
Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following unique BioBrick combinations:
| Combination # | Fluorescent Protein BioBrick | Fluorescent Protein | Promoter/RBS BioBrick | Promoter;RBS Strength |
| 1 | BBa_K592100 | BFP | BBa_K608004 | Strong; Weak |
| 2 | BBa_K592100 | BFP | BBa_K608007 | Medium; Weak |
| 3 | BBa_E0020 | ECFP | BBa_K608007 | Medium; Weak |
| 4 | BBa_E0020 | ECFP | BBa_K608003 | Strong; Medium |
| 5 | BBa_E0020 | ECFP | BBa_K608002 | Strong; Strong |
| 6 | BBa_K592101 | YFP | BBa_K608002 | Strong; Strong |
| 7 | BBa_K592101 | YFP | BBa_K608006 | Medium; Medium |
| 8 | BBa_K592101 | YFP | BBa_K608007 | Medium; Weak |
| 9 | BBa_K864100 | SYFP2 | BBa_K608002 | Strong; Strong |
| 10 | BBa_K864100 | SYFP2 | BBa_K314100 | Strong; Very Strong |
| 11 | BBa_K864100 | SYFP2 | BBa_K608006 | Medium; Medium |
| 12 | BBa_E0030 | EYFP | BBa_K608007 | Medium; Weak |
| 13 | BBa_EE030 | EYFP | BBa_K608006 | Medium; Medium |
| 14 | BBa_E0030 | EYFP | BBa_K314100 | Strong; Very Strong |
plasmids transformed by electroporation into pSB1c3
Several fluorescent protein/promoter+RBS combinations were studied in more than one experiment, which is why each is classified as a 'unique' combination.</br>
Each BioBrick combination was
Fluorescence Readings
relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)
Protocol