Difference between revisions of "Team:Freiburg/Protocols/TerrificBroth"
Line 39: | Line 39: | ||
<hr/> | <hr/> | ||
</div> | </div> | ||
− | <h4> | + | <h4>Terrific Broth</h4> |
<div class="level4"> | <div class="level4"> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
<div class="table sectionedit2"><table class="inline"> | <div class="table sectionedit2"><table class="inline"> | ||
<tr class="row0"> | <tr class="row0"> | ||
− | <th class="col0"> | + | <th class="col0">Component </th><th class="col1"> for 1 litre medium </th><td class="col2"> <b>remarks</b> </td> |
</tr> | </tr> | ||
<tr class="row1"> | <tr class="row1"> | ||
− | < | + | <td class="col0"> Monopotassium phosphate </td><td class="col1"> 5.6 g </td><td class="col2"> </td> |
− | + | ||
<tr class="row2"> | <tr class="row2"> | ||
− | <td class="col0"> | + | <td class="col0"> Potassium phosphate dibasic </td><td class="col1"> 28.9 g </td><td class="col2"> </td> |
− | + | ||
<tr class="row3"> | <tr class="row3"> | ||
− | <td class="col0"> | + | <td class="col0"> yeast extract </td><td class="col1"> 10 </td> |
</tr> | </tr> | ||
<tr class="row4"> | <tr class="row4"> | ||
− | <td class="col0"> | + | <td class="col0"> Thiamine </td><td class="col1"> 10 mg </td><td class="col2"> added after sterilisation (filter sterilized) </td> |
+ | </tr> | ||
+ | <tr class="row5"> | ||
+ | <td class="col0"> Glucose (20%) </td><td class="col1"> 40ml </td><td class="col2"> added after sterilisation (filter sterilized) </td> | ||
</tr> | </tr> | ||
</table></div> | </table></div> | ||
− | <!-- EDIT2 TABLE [ | + | <!-- EDIT2 TABLE [412-920] --> |
− | <li class="level1"><div class="li"> | + | </div> |
+ | <li class="level1"><div class="li"> autoclave medium than add filter sterilized Thiamine and Glucose</div> | ||
</li> | </li> | ||
− | <li class="level1"><div class="li"> | + | <li class="level1"><div class="li"> Store at 4°C</div> |
<ol> | <ol> | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<div class="tags"><span> | <div class="tags"><span> | ||
<a class="wikilink1" href="/igem2015/doku.php?id=tag:protocols&do=showtag&tag=protocols" rel="tag" title="tag:protocols">protocols</a>, | <a class="wikilink1" href="/igem2015/doku.php?id=tag:protocols&do=showtag&tag=protocols" rel="tag" title="tag:protocols">protocols</a>, |
Latest revision as of 22:07, 14 September 2015
![](https://static.igem.org/mediawiki/2015/8/85/Freiburg_wiki_background_2_parted_left.png)
![](https://static.igem.org/mediawiki/2015/f/f4/Freiburg_wiki_background_2_parted_right.png)
Preparation of Teriffic Broth
Terrific Broth
Component | for 1 litre medium | remarks |
---|---|---|
Monopotassium phosphate | 5.6 g | |
Potassium phosphate dibasic | 28.9 g | |
yeast extract | 10 | |
Thiamine | 10 mg | added after sterilisation (filter sterilized) |
Glucose (20%) | 40ml | added after sterilisation (filter sterilized) |
autoclave medium than add filter sterilized Thiamine and Glucose
Store at 4°C