Difference between revisions of "Team:UMaryland/Interlab"

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<h1><b>Protocols</b></h1>
 
<h1><b>Protocols</b></h1>
 
<p style="font-size:20px">Protocols for construction of devices here.</p>
 
<p style="font-size:20px">Protocols for construction of devices here.</p>
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<div class = "text" align = "left">
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DNA Kit Plate Instructions  (http://parts.igem.org/Help:2015_DNA_Distribution)
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                        <br>
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Transformation (http://parts.igem.org/Help:Protocols/Transformation)
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                        <br>
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Preparing overnight cultures for testing
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<br>
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• Prepare culture in a 10 mL culture tube with a loose top
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<br>
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• Add 5mL LB using a seriological pipette
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<br>
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• Add 5uL of 1000x antibiotic (Chloramphenicol.)
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<br>
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• Pick a distinct colony using a pipette tip and eject tip into tube.
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<br>
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<a href = "http://www.hawaii.edu/microbiology/MO/docs/diversity/Qia-Miniprep.pdf">Miniprep – using Qiagen protocol</a>
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<br>
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<a href = "http://parts.igem.org/Help:Protocols/Transformation">Transformation</a>
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<br>
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<a href = "https://www.neb.com/~/media/NebUs/Files/Application%20Notes/appNoteM0486.pdf">Colony PCR (selecting transformants from the transformation plates)</a>
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Revision as of 21:51, 14 September 2015