material: chemicals, used kits, …
duration: 90 min

• All fluorescence measurements were performed with a microplate reader in blach plates with transparent bottom
• As Exitation wavelength 480nm were choosen
• Emission was measured at 520nm or a full spectrum was taken from 500 to 700 nm
• Reactions were either performed in a tube and transferred to the microplate after the cell-free reaction or directly performed inside the platereader
• when the reaction was performed inside the Platreader the wells were covered with foil to avoid evaporation
• After the reaction and Measurement samples were stored at 4°C for further folding or directly frozen at -20°C
• for a ensurence of sucessful expression of GFP an Westernblot was performed