Difference between revisions of "Team:UMaryland/Design"
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<li>1. Hok-Sok was coupled to a constitutive generator of unstable RFP to form a larger composite part.</li> | <li>1. Hok-Sok was coupled to a constitutive generator of unstable RFP to form a larger composite part.</li> | ||
<li>2. Unstable LVA-tagged RFP has a half-life of 1 hour and allows for more current measurements of protein production.</li> | <li>2. Unstable LVA-tagged RFP has a half-life of 1 hour and allows for more current measurements of protein production.</li> | ||
− | <li>3. | + | <li>3. For our first testing method to see if Hok-Sok was capable of maintaining a plasmid, we wanted to measure how RFP fluorescence was retained over many generations in two <i>E. coli</i> strains: BL21 and DH5α.</li> |
− | <li>4. | + | <li>4. In order to test, five sets of cultures were grown in LB media for fluorescence studies.</li> |
<ol> | <ol> | ||
<li>A. Constitutive unstable RFP grown <b>with</b> chloramphenicol (33 µg/mL) in media <b>(+ Control)</b></li> | <li>A. Constitutive unstable RFP grown <b>with</b> chloramphenicol (33 µg/mL) in media <b>(+ Control)</b></li> |
Revision as of 17:43, 15 September 2015