Difference between revisions of "Team:BGU Israel/Experiments"

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<h2>Experiments &amp; Protocols</h2>
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
 
  
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<h5>What should this page contain?</h5>
 
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<ul>
 
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<li> Protocols </li>
 
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<li> Experiments </li>
 
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<li>Documentation of the development of your project </li>
 
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</ul>
 
  
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<h2> Those are the protocols we used throughout the summer:</h2>
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        <table width=90% align=center class="parts">
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<tr><br><br><br>
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<td><h3><b>Protocol details</b></h3></td>
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<td><h3><b>Link to Protocol</b></h3></td>
  
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<h4>Inspiration</h4>
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</tr>
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<ul>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<tr>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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<td>cell transformation details</td>
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</ul>
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<td><a href="https://static.igem.org/mediawiki/2015/7/76/BGU_PROTOCOLS_cell_transform.pdf"> cell transformation</a></td>
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</div>  <br /><br /><br /> <br /><br /><br />
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</tr>
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<tr>
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<td>Gel Extraction details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/4/4a/Gel_extraction.pdf"> Gel Extraction </a></td>
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</tr>
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<!--1-->
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<tr>
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<td> Ligation details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/9/9d/Ligation.pdf"> Ligation </a></td>
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</tr>
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<tr>
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<td>Midiprep  </td>
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<td><a href="https://static.igem.org/mediawiki/2015/4/47/Midiprep.pdf"> Midiprep </a></td>
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</tr>
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<tr>
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<td>Miniprep details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/d/dd/Miniprep.pdf"> Miniprep</a></td>
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</tr>
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<tr>
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<td>PCR_hifi details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/9/9e/PCR_hifi.pdf"> PCR_hifi</a></td>
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</tr>
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<tr>
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<td>PCR Purification details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/b/b7/Vilnius15_PCRPurification.pdf">PCR Purification</a></td>
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</tr>
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<tr>
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<td>Virus details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/e/e7/Virus.pdf"> Virus</a></td>
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</tr>
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<tr>
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<td>AAV_production_Protocol details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/d/dd/AAV_production_Protocol.pdf"> AAV_production_Protocol</a></td>
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</tr>
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<tr>
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<td>CaP_transfection details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/8/89/CaP_transfection.pdf">CaP_transfection</a></td>
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</tr>
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<tr>
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<td>EZ-RNA_Toal_RNA_isolation details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/b/b0/EZ-RNA_Toal_RNA_isolation_Kit.pdf">EZ-RNA_Toal_RNA_isolation</a></td>
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</tr>
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<tr>
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<td>High_Capacity_cDNA_reverse_transcription details</td>
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<td><a href="https://static.igem.org/mediawiki/2015/f/fd/High_Capacity_cDNA_reverse_transcription_kit.pdf">High_Capacity_cDNA_reverse_transcription</a></td>
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</tr>
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<tr>
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<td>Gel Preparation details </td>
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<td><a href="https://static.igem.org/mediawiki/2015/c/ca/Gel_preparation.pdf"> Gel Preparation </a></td>
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</tr>
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            <tr>
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<td>Preparation of anibiotics stocks details </td>
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<td><a href="https://static.igem.org/mediawiki/2015/c/cf/Preparation_of_anibiotics_stocks.pdf"> Preparation of anibiotics stocks
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  </a></td>
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</tr>
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            <tr>
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<td>Preparation of LB Agar plates details </td>
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<td><a href="https://static.igem.org/mediawiki/2015/f/f4/Preparation_of_LB_Agar_plates.pdf"> Preparation of LB Agar plates </a></td>
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</tr>
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            <tr>
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<td>Preparation of liquid LB (for bacteria growth) details </td>
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<td><a href="https://static.igem.org/mediawiki/2015/6/65/Preparation_of_liquid_LB.pdf"> Preparation of liquid LB (for bacteria growth) </a></td>
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</tr>
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</table>
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<br /><br /><br /><br />
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        <!-- end of content -->
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  <br /><br /><br />
  
  

Revision as of 15:34, 15 September 2015

Team:BGU Israel



Those are the protocols we used throughout the summer:




Protocol details

Link to Protocol
cell transformation details cell transformation
Gel Extraction details Gel Extraction
Ligation details Ligation
Midiprep Midiprep
Miniprep details Miniprep
PCR_hifi details PCR_hifi
PCR Purification details PCR Purification
Virus details Virus
AAV_production_Protocol details AAV_production_Protocol
CaP_transfection details CaP_transfection
EZ-RNA_Toal_RNA_isolation details EZ-RNA_Toal_RNA_isolation
High_Capacity_cDNA_reverse_transcription details High_Capacity_cDNA_reverse_transcription
Gel Preparation details Gel Preparation
Preparation of anibiotics stocks details Preparation of anibiotics stocks
Preparation of LB Agar plates details Preparation of LB Agar plates
Preparation of liquid LB (for bacteria growth) details Preparation of liquid LB (for bacteria growth)