Difference between revisions of "Team:NYU Shanghai/Protocols"
Line 386: | Line 386: | ||
<td>0.00438 g</td> | <td>0.00438 g</td> | ||
<td>0.08766 g</td> | <td>0.08766 g</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | Lysozyme Solution (Desired Total Volume: 15mL)<br> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>Lysozyme Solubility: </td> | ||
+ | <td>10 mg lysozyme in 1 mL of 10 mM Tris-HCl</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Desired Amount of Lysozyme Solution to Make:</td> | ||
+ | <td>15 mL</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Amount of Lysozyme Needed:</td> | ||
+ | <td>10 mg x 15 = <b>150 mg</b></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Amount of 10 mM Tris-HCl Needed: </td> | ||
+ | <td><b>15 mL</b></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | Reagent Solution (Desired total volume: 22 mL)<br> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>Chemical Name:</td> | ||
+ | <td>ATP disodium salt trihydrate</td> | ||
+ | <td>MgSO4•7H2O</td> | ||
+ | <td>HEPES Buffer</td> | ||
+ | <td>D-Luciferin free acid</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Molecular Weight:</td> | ||
+ | <td>605.24 g/mol</td> | ||
+ | <td>246.5 g/mol</td> | ||
+ | <td>238.3 g/mol</td> | ||
+ | <td>280.33 g/mol</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Molarity Desired:</td> | ||
+ | <td>3 mM</td> | ||
+ | <td>15 mM</td> | ||
+ | <td>30 mM</td> | ||
+ | <td>1 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Calculation:</td> | ||
+ | <td> </td> | ||
+ | <td> </td> | ||
+ | <td> </td> | ||
+ | <td>Use the 1 mM stock solution created earlier</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Final Amount:</td> | ||
+ | <td>0.039945 g</td> | ||
+ | <td>0.081345 g</td> | ||
+ | <td>0.1573 g</td> | ||
+ | <td>22 mL</td> | ||
</tr> | </tr> | ||
</table> | </table> |
Revision as of 06:13, 16 September 2015