Difference between revisions of "Team:Freiburg/Project/Overview"
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− | <h1> | + | <h1>Surface chemistry</h1> |
− | <p> | + | <p>The production of a protein microarray in the DiaCHIP is based on specific immobilization of antigenic peptides on a glass slide. Therefore, we optimized a protocol for a specific surface chemistry until the proportion of unspecific binding of non-target proteins was reduced to a minimum.<br> |
+ | <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/Surface_Chemistry">Read more</a> about the different systems we tested on our way to high specificity. | ||
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Revision as of 13:42, 16 September 2015
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The DiaCHIP - A Versatile Detection System
![DiaCHIP_Sabi](https://static.igem.org/mediawiki/2015/a/af/Freiburg_DiaCHIP_Sabi.png)
Project Motivation
Serological tests are a key element in modern medicine. Especially for detection and identification of infectious diseases, performing several blood tests is inevitable. Testing for more than one disease at a time or diagnosing a patient with diffuse symptoms usually requires more than one serological test. Every single test that is performed increases the time of waiting for a result as well as the bill. In case of dangerous infectious diseases every minute until the onset of the appropriate treatment is important for life. What if there was a possibility to combine all this testing in one single device which is affordable for everyone?
Detecting Antigen-Antibody Interactions
The DiaCHIP is an innovative tool to screen for a broad range of antibodies present in serum. Antibodies serve indicator for an immune response towards an infection or a successful vaccination. They also play an important role in the diagnosis of autoimmune diseases. Spotting diseases by detecting correspondent antibodies in a patient's serum is an established method in modern diagnostics.
Based on the very same principle, the DiaCHIP enables to simultaneously screen for multiple diseases at time, thereby reducing time and cost of a diagnosis. Especially the ability to differentiate between life threatening diseases and mild infections within a short time bears the potential to save lives.
The Concept
The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis and a novel method for label-free detection packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM Teams.