Difference between revisions of "Team:Goettingen/Experiments"

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<h2> Protein extraction and Purification</h2>
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<a href="" onClick=" $('#menu29').slideToggle(300, function callback() {  }); return false;"><h1 style="color:white;"> Protein Extraction (French Press) and Purification (Protino® Ni-IDA His-Tag protein purification, Macherey-Nagel)</h1></a>
 
<a href="" onClick=" $('#menu29').slideToggle(300, function callback() {  }); return false;"><h1 style="color:white;"> Protein Extraction (French Press) and Purification (Protino® Ni-IDA His-Tag protein purification, Macherey-Nagel)</h1></a>
 
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<h2> Restriction controls </h2>
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<h2> Restriction Controls </h2>
 
                     <a href="" onClick=" $('#menu17').slideToggle(300, function callback() {  }); return false;"><h1> Aan I (Psi I ) - thermo fisher scientific - restriction control protocol</h1></a>
 
                     <a href="" onClick=" $('#menu17').slideToggle(300, function callback() {  }); return false;"><h1> Aan I (Psi I ) - thermo fisher scientific - restriction control protocol</h1></a>
 
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<h2> PCR preparation methods </h2>
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<h2> PCR Preparation Methods </h2>
 
                     <a href="" onClick=" $('#menu23').slideToggle(300, function callback() {  }); return false;"><h1> Colony PCR</h1></a>
 
                     <a href="" onClick=" $('#menu23').slideToggle(300, function callback() {  }); return false;"><h1> Colony PCR</h1></a>
 
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Revision as of 17:18, 16 September 2015



Media

LB Medium

"Fat" LB Medium

Phosphatase Activity plates, Sperber media

Esterase Activity plates, with 1% Tributyrin

Cellulase activity plates

Cloning Methods

PCR product purification using QIAquick® PCR Purification Kit (QIAGEN)

PCR Gel extraction, peqGOLD Gel Extraction Kit

Blunt End Ligation in pJET1.2 vector –Clone JET PCR Cloning Kit–Thermo Scientific

TOPO® Cloning protocol usingChampion™ pET Directional TOPO® Expression Kits. Thermo Fisher Scientific

Plasmid transformation into chemically competent E. coli

Electroporation of BL21 cells with pJET_RFP

Plasmid Extraction - using QIAprep Spin Miniprep Kit (QIAGEN)

Plasmid Extraction - using peqGOLD Plasmid Miniprep Kit I (PEQLAB Technologies)

Competent Cells

Preparation of competent E.coli cells

Transformation Efficiency Kit, RFP construct (iGEM)

Protein Extraction and Purification

Protein Extraction (French Press) and Purification (Protino® Ni-IDA His-Tag protein purification, Macherey-Nagel)