Wetlab
EcnB part extracted from A(ABEF) synthesized plasmids and ligated with H backbone (pSB1C3). Parts were column purified, ligated, then transformed. Had some issues growing cultures with ligations. We are excited to start cloning!

Drylab
Dry lab continued with the CAD design of the fish tag. We also did research on a list of potentially compatible materials for the fish tag, including tubing, clamps, and syringes.

Outreach
The YOURS kids got a tour of our lab and we taught them more about how DNA works in our body as the determining factor of our genetic characteristics. They were able to place their fingers on plates to “plate bacteria” and we showed them how to incubate the plates to help the bacteria grow. We helped them carry out an experiment that extracted DNA from strawberries and we showed them proper pipetting techniques. We also did a fun experiment with the kids with basic cooking ingredients to make a “volcano explode” to introduce chemical reactions.
We narrowed our search to a few fish hatcheries in the upstate New York region and began contacting them.