Difference between revisions of "Team:Cornell/notebook"

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                                                               <b> Drylab</b> <br> Dry lab continued with the CAD design of the fish tag. We also did research on a list of potentially compatible materials for the fish tag, including tubing, clamps, and syringes. <br> <br> <br> <br>
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                                                               <b> Drylab</b> <br> Dry lab discussed and finalized a design as well as the dimensions for the fish tag. Parts were designed through Solidworks in member’s own time.  <br> <br> <br> <br>
  
 
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                                                               <b> Outreach</b> <br> The YOURS kids got a tour of our lab and we taught them more about how DNA works in our body as the determining factor of our genetic characteristics. They were able to place their fingers on plates to “plate bacteria” and we showed them how to incubate the plates to help the bacteria grow. We helped them carry out an experiment that extracted DNA from strawberries and we showed them proper pipetting techniques. We also did a fun experiment with the kids with basic cooking ingredients to make a “volcano explode” to introduce chemical reactions. <br>
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                                                               <b> Outreach</b> <br> We began searching for fish farm contacts, and started our YOURS mentorship program. We introduced our mentors to the kids and taught them about what DNA is.
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  <br> <br> <br> <br>
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We narrowed our search to a few fish hatcheries in the upstate New York region and began contacting them.   <br> <br> <br> <br>
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<h1 id = "w2">Week 2</h1>
 
<h1 id = "w2">Week 2</h1>
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                            <img src="https://static.igem.org/mediawiki/2015/5/5d/Cornell_flavoLogoSml.png" style="margin-left:33px">
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                                                              <b> Wetlab </b> <br> Week one steps were redone. Started gel purifications instead of column purifications, so plasmids were re-cultured (pre-ligation). We had some issues with low DNA concentrations after gel purifications, so we increased amount of DNA used in digestions.<br>
 +
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<i>Flavo Subteam</i><br>
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The flavo subteam made cultures of two strains of Flavobacterium, and measured growth of bacteria with spectrophotometer. In addition, daily OD readings were taken to determine growth rate.
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<br> <br> <br> <br>
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                            <img src="https://static.igem.org/mediawiki/2015/5/55/Cornell_fishBitLogoSml.png" style="margin-left:20px">
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                                                        <div class = "col-md-10">
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                                                              <b> Drylab</b> <br>Dry lab continued with the CAD design of the fish tag. We also did research on a list of potentially compatible materials for the fish tag, including tubing, clamps, and syringes.
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.  <br> <br> <br> <br>
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                            <img src="https://static.igem.org/mediawiki/2015/d/d5/Cornell_outreachLogoSml.png" style="margin-left:30px">
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                                                        <div class = "col-md-10">
 +
                                                              <b> Outreach</b> <br> The YOURS kids got a tour of our lab and we taught them more about how DNA works in our body as the determining factor of our genetic characteristics. They were able to place their fingers on plates to “plate bacteria” and we showed them how to incubate the plates to help the bacteria grow. We helped them carry out an experiment that extracted DNA from strawberries and we showed them proper pipetting techniques. We also did a fun experiment with the kids with basic cooking ingredients to make a “volcano explode” to introduce chemical reactions. <br>
 +
 +
We narrowed our search to a few fish hatcheries in the upstate New York region and began contacting them.  <br> <br> <br> <br>
 +
 +
 +
                                                        </div>
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</div>
 
</div>

Revision as of 00:44, 17 September 2015

Cornell iGEM

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Overview

Week 1

Wetlab
EcnB part extracted from A(ABEF) synthesized plasmids and ligated with H backbone (pSB1C3). Parts were column purified, ligated, then transformed. Had some issues growing cultures with ligations. We are excited to start cloning!



Drylab
Dry lab discussed and finalized a design as well as the dimensions for the fish tag. Parts were designed through Solidworks in member’s own time.



Outreach
We began searching for fish farm contacts, and started our YOURS mentorship program. We introduced our mentors to the kids and taught them about what DNA is.



Week 2

Wetlab
Week one steps were redone. Started gel purifications instead of column purifications, so plasmids were re-cultured (pre-ligation). We had some issues with low DNA concentrations after gel purifications, so we increased amount of DNA used in digestions.
Flavo Subteam
The flavo subteam made cultures of two strains of Flavobacterium, and measured growth of bacteria with spectrophotometer. In addition, daily OD readings were taken to determine growth rate.



Drylab
Dry lab continued with the CAD design of the fish tag. We also did research on a list of potentially compatible materials for the fish tag, including tubing, clamps, and syringes. .



Outreach
The YOURS kids got a tour of our lab and we taught them more about how DNA works in our body as the determining factor of our genetic characteristics. They were able to place their fingers on plates to “plate bacteria” and we showed them how to incubate the plates to help the bacteria grow. We helped them carry out an experiment that extracted DNA from strawberries and we showed them proper pipetting techniques. We also did a fun experiment with the kids with basic cooking ingredients to make a “volcano explode” to introduce chemical reactions.
We narrowed our search to a few fish hatcheries in the upstate New York region and began contacting them.



Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15