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Chromoproteins
Pigmented compounds that absorb and reflect visible light.
Fluorescent Proteins
Pigmented compounds that absorb UV light and emit visible light.
Luciferase Enzymes
Catalyzes conversion of luciferin to oxyluciferin. Energy is released in the form of the light.
We created constructs to express 3 different colors of chromoproteins: blue, lime, and yellow. These were taken from the registry, created in 2010 by the Cambridge iGEM team.
| BBa_K880005 | promoter/rbs |
| BBa_K081005 | promoter/rbs |
| BBa_K1333315 | pBAD with araC |
| BBa_K592009 | amilCP Blue |
| BBa_K1467201 | Golden Gate compatible amilCP blue |
| BBa_K1033910 | fwYellow Chromoprotein |
| BBa_K1033916 | amajLime yellow-green chromoprotein |
| BBa_B0024 | terminator |
| BBa_B0011 | terminator |
XJTLU team used the T7 promoter in a bl21 E. Coli. This produced good blue and yellow color in our lab.
We utilized generators to express 2 different colors of fluorescent proteins: RFP and GFP. These parts from the registry.
| BBa_J04450 | RFP |
| BBa_J04451 | RFP |
| BBa_KI13600 | CFP |
| BBa_KE0040 | GFP |
We utilized generators to express 4 different bioluminescent colors: red, green, yellow, and orange.
| BBa_K325219 | Red Firefly Luciferase and LRE (under pBAD) |
| BBa_K325209 | Green Firefly Luciferase and LRE (under pBAD) |
| BBa_K325259 | Yellow Firefly Luciferase (under pBAD) |
| BBa_K325218 | Orange Firefly Luciferase (under pBAD) |
| BBa_K1486022 | Renilla Luciferase |
| BBa_K325108 | EPIC Firefly Luciferase (under pBAD) |
To create a natural beat generator, we created a light oscillating system. Unfortunately, the time scale was far too long for any practical beat generator (hypothetical 40min per beat). See Fudan 2012 Biowave for more information.
