Difference between revisions of "Team:BABS UNSW Australia/Protocols"

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                 <a href="#introduction">Protocols</a>
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Revision as of 06:56, 17 September 2015

Protocols

Given the differing levels of experience in the team, and the plethora of protocols available on the net we decided to standardise our protocols and keep them in a tidy little orange folder. Here are most of the protocols we used over year including our standard template

Template

  • Babs UNSW iGEM protocol template

    • Media and Solutions

  • LB Broth Preparation
  • LB Agar Preparation
  • Agar Plate Preparation
  • Antibiotic Preparation
  • Streptococcal Regeneration Media
  • GM17 Media Preparation
  • SOC Media Preparation
  • Preparation of Tris-HCL Buffer
  • 1M Na2CO3

    • DNA Manipulation

  • Enzyme Digestion
  • Ligation iGEM protocol
  • Ligation iGEM protocol V2
  • Gel Electrophoresis

    • Transformations

  • E.coli Transformation
  • Preparation of Lactococcus Competent Cells
  • Lactococcus electrotransformation pre-treatement solution
  • Simple Lactococcus transformation
  • Lactococcus Transformation V1
  • Lactococcus Transformation V2

    • Other

  • Miniprep iGEM protocol
  • PCR purification iGEM protocol
  • Overlap PCR
  • Sequencing preparation iGEM Protocol
  • Mammalian Cell assay