Difference between revisions of "Team:NYU Shanghai/Protocols"
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<p>Link to <a href="https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202"> NEB Protocol </a></p> | <p>Link to <a href="https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202"> NEB Protocol </a></p> | ||
<ol> | <ol> | ||
− | <li>Make Reaction mixture</li> | + | <li>Make Reaction mixture</li><br> |
<table border="1"> | <table border="1"> | ||
<tr style="font-weight: bold;"> | <tr style="font-weight: bold;"> | ||
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<li>Reaction mixture with no insert DNA</li> | <li>Reaction mixture with no insert DNA</li> | ||
<li>Reaction mixture with no insert DNA and no ligase</li> | <li>Reaction mixture with no insert DNA and no ligase</li> | ||
− | </ul> | + | </ul><br> |
<li>Ligation temperature and times vary</li> | <li>Ligation temperature and times vary</li> | ||
− | <table border="1"> | + | <table border="1" width="450"> |
<tr> | <tr> | ||
<td>For inserting a part into backbone (no 3A assembly), the suggested NEB protocol worked</td> | <td>For inserting a part into backbone (no 3A assembly), the suggested NEB protocol worked</td> | ||
Line 616: | Line 616: | ||
</tr> | </tr> | ||
</table> | </table> | ||
− | <li>Heat inactivate at 65°C for 10 minutes.</li> | + | <li>Heat inactivate at 65°C for 10 minutes.</li><br> |
<li>Chill on ice and transform 1-5 μl of the reaction into 50 μl competent cells.</li> | <li>Chill on ice and transform 1-5 μl of the reaction into 50 μl competent cells.</li> | ||
</ol> | </ol> |
Revision as of 11:53, 17 September 2015