Difference between revisions of "Team:Freiburg/Labjournals/Plasmids"

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<div class="table sectionedit1"><table class="inline">
 
<div class="table sectionedit1"><table class="inline">
 
<tr class="row0">
 
<tr class="row0">
<th class="col0"> No. </th><th class="col1"> Signature </th><th class="col2 rightalign">  C-term. Tag </th><th class="col3"> Antigen </th><th class="col4"> N-term. Tag </th><th class="col5"> Resistence </th><th class="col6"> Purpose </th>
+
<th class="col0"> No. </th><th class="col1"> Signature </th><th class="col2 rightalign">  C-term. Tag </th><th class="col3"> Antigen </th><th class="col4"> N-term. Tag </th><th class="col5"> Resistence </th><th class="col6"> Purpose </th><th class="col7">GenBank file</th>
 
</tr>
 
</tr>
 
<tr class="row5">
 
<tr class="row5">
<td class="col0"> 5 </td><td class="col1"> pET_603 </td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
+
<td class="col0"> 5 </td><td class="col1"><a class="lightbox_trigger" href="https://static.igem.org/mediawiki/2015/7/7e/Freiburg_pET_603.jpg" width="400px">pET_603</a>  </td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
glycoprotein E1 </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
+
glycoprotein E1 </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td><td class="col7"><a class="media" href="https://static.igem.org/mediawiki/2015/8/81/Freiburg_pET_603GB.gb">pET_603.gb</a></td>
 
</tr>
 
</tr>
 
<tr class="row6">
 
<tr class="row6">
<td class="col0"> 6 </td><td class="col1"> pRIG15_06 </td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
+
<td class="col0"> 6 </td><td class="col1"> <a class="lightbox_trigger" href="https://static.igem.org/mediawiki/2015/8/87/Freiburg_pRIG15_6.jpg" width="400px">pRIG15_6</a></td><td class="col2"> - </td><td class="col3"> Rubella Virus, <br/>
glycoprotein E1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
+
glycoprotein E1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td><td class="col7"><a class="media" href="https://static.igem.org/mediawiki/2015/e/eb/Freiburg_pRIG15_6_GB.gb">pRIG15_6.gb</a></td>
 
</tr>
 
</tr>
 
<tr class="row8">
 
<tr class="row8">
<td class="col0"> 8 </td><td class="col1"> pRIG15_07 </td><td class="col2"> - </td><td class="col3"> Human Herpes Virus, <br/>
+
<td class="col0"> 8 </td><td class="col1"> <a class="lightbox_trigger" href="https://static.igem.org/mediawiki/2015/3/3e/Freiburg_pRIG15_7.jpg" width="400px">pRIG15_7</a></td><td class="col2"> - </td><td class="col3"> Human Herpes Virus, <br/>
glycoprotein E </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
+
glycoprotein E </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td><td class="col7"> <a class="media" href="https://static.igem.org/mediawiki/2015/6/68/Freiburg_pRIG15_7_GB.gb">pRIG15_7.gb</a></td>
 
</tr>
 
</tr>
 
<tr class="row10">
 
<tr class="row10">
<td class="col0"> 10 </td><td class="col1"> pIG15_804 </td><td class="col2"> 10xHis </td><td class="col3"> Herpes simplex, <br/>
+
<td class="col0"> 10 </td><td class="col1"> <a class="lightbox_trigger" href="" width="400px">pIG15_804</a> </td><td class="col2"> 10xHis </td><td class="col3"> Herpes simplex, <br/>
immunodominant epitope of glycoprotein G1 </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
+
immunodominant epitope of glycoprotein G1 </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td><td class="col7" <a class="media" href=""></a></td>
 
</tr>
 
</tr>
 
<tr class="row11">
 
<tr class="row11">
<td class="col0"> 11 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_803.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_803.pdf">pET_803</a> </td><td class="col2"> - </td><td class="col3"> Herpes simplex, <br/>
+
<td class="col0"> 11 </td><td class="col1"> <a class="lightbox_trigger" href="https://static.igem.org/mediawiki/2015/b/b8/Freiburg_pET_803.jpg" width="400px">pET_803</a> </td><td class="col2"> - </td><td class="col3"> Herpes simplex, <br/>
immunodominant epitope of glycoprotein G1 </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
+
immunodominant epitope of glycoprotein G1 </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td><td class="col7"><a class="media" href="https://static.igem.org/mediawiki/2015/8/81/Freiburg_pET_803_GB.gb">pET_803.gb</a></td>
 
</tr>
 
</tr>
 
<tr class="row12">
 
<tr class="row12">
<td class="col0"> 12 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:pet_804.pdf" class="media mediafile mf_pdf" title="labjournal:pet_804.pdf">pET_804</a> </td><td class="col2"> 6xHis </td><td class="col3"> Herpes simplex, <br/>
+
<td class="col0"> 12 </td><td class="col1"> <a class="lightbox_trigger" href="https://static.igem.org/mediawiki/2015/a/a5/Freiburg_pET_804.jpg" width="400px">pET_804</a> </td><td class="col2"> 6xHis </td><td class="col3"> Herpes simplex, <br/>
immunodominant epitope of glycoprotein G1 </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
+
immunodominant epitope of glycoprotein G1 </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td><td class="col7"><a class="media" href="https://static.igem.org/mediawiki/2015/2/24/Freiburg_pET_804_GB.gb">pET_804.gb</a></td>
 
</tr>
 
</tr>
 
<tr class="row13">
 
<tr class="row13">
<td class="col0"> 13 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:prig15_8.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:prig15_8.pdf">pRIG15_08</a> </td><td class="col2"> - </td><td class="col3"> Herpes simplex, <br/>
+
<td class="col0"> 13 </td><td class="col1"> <pRIG15_08 </td><td class="col2"> - </td><td class="col3"> Herpes simplex, <br/>
 
immunodominant epitope of glycoprotein G1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
immunodominant epitope of glycoprotein G1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
</tr>
 
<tr class="row14">
 
<td class="col0"> 14 </td><td class="col1"> pIG15_901 </td><td class="col2"> - </td><td class="col3"> Yellow Fever Virus, <br/>
 
non-structural protein 1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Cloning </td>
 
</tr>
 
<tr class="row15">
 
<td class="col0"> 15 </td><td class="col1"> pIG15_1001 </td><td class="col2"> - </td><td class="col3"> Hepatits C, <br/>
 
core protein </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
</tr>
 
</tr>
 
<tr class="row16">
 
<tr class="row16">
<td class="col0"> 16 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1003.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1003.pdf">pET_1003</a> </td><td class="col2"> - </td><td class="col3"> Hepatits C, <br/>
+
<td class="col0"> 16 </td><td class="col1"> pET_1003 </td><td class="col2"> - </td><td class="col3"> Hepatits C, <br/>
 
core protein </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
core protein </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row17">
 
<tr class="row17">
<td class="col0"> 17 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1004.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1004.pdf">pET_1004</a> </td><td class="col2"> 6xHis </td><td class="col3"> Hepatits C, <br/>
+
<td class="col0"> 17 </td><td class="col1"> pET_1004 </td><td class="col2"> 6xHis </td><td class="col3"> Hepatits C, <br/>
 
core protein </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
core protein </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
</tr>
 
<tr class="row18">
 
<td class="col0"> 18 </td><td class="col1"> pIG15_1101 </td><td class="col2"> - </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
 
TeNT_Hc </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
</tr>
 
</tr>
 
<tr class="row19">
 
<tr class="row19">
<td class="col0"> 19 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pig15_1104.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pig15_1104.pdf">pIG15_1104</a> </td><td class="col2"> 10xHis </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
<td class="col0"> 19 </td><td class="col1"> pIG15_1104 </td><td class="col2"> 10xHis </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
TeNT_Hc </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
+
</tr>
+
<tr class="row20">
+
<td class="col0"> 20 </td><td class="col1"> pIG15_1104_HA </td><td class="col2"> 10xHis_HA </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
 
TeNT_Hc </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
TeNT_Hc </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row21">
 
<tr class="row21">
<td class="col0"> 21 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pig15_1105.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pig15_1105.pdf">pIG15_1105</a> </td><td class="col2"> 10xHis </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
<td class="col0"> 21 </td><td class="col1"> pIG15_1105 </td><td class="col2"> 10xHis </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
 
TeNT_Hc </td><td class="col4"> Halo </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
TeNT_Hc </td><td class="col4"> Halo </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row22">
 
<tr class="row22">
<td class="col0"> 22 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1103.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1103.pdf">pET_1103</a> </td><td class="col2"> - </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
<td class="col0"> 22 </td><td class="col1"> pET_1103 </td><td class="col2"> - </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
 
TeNT_Hc </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
TeNT_Hc </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row23">
 
<tr class="row23">
<td class="col0"> 23 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1104.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1104.pdf">pET_1104</a> </td><td class="col2"> 6xHis </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
<td class="col0"> 23 </td><td class="col1"> pET_1104 </td><td class="col2"> 6xHis </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
TeNT_Hc </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
+
</tr>
+
<tr class="row24">
+
<td class="col0"> 24 </td><td class="col1"> pET_1104_HA </td><td class="col2"> 6xHis_HA </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
 
TeNT_Hc </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
TeNT_Hc </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row25">
 
<tr class="row25">
<td class="col0"> 25 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1105.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1105.pdf">pET_1105</a> </td><td class="col2"> 6xHis</td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
<td class="col0"> 25 </td><td class="col1"> pET_1105 </td><td class="col2"> 6xHis</td><td class="col3"> <em>Clostridium tetani</em>, <br/>
 
TeNT_Hc </td><td class="col4"> Halo </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
TeNT_Hc </td><td class="col4"> Halo </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row26">
 
<tr class="row26">
<td class="col0"> 26 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:prig15_11.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:prig15_11.pdf">pRIG15_11</a> </td><td class="col2"> - </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
+
<td class="col0"> 26 </td><td class="col1"> pRIG15_11 </td><td class="col2"> - </td><td class="col3"> <em>Clostridium tetani</em>, <br/>
 
TeNT_Hc </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
TeNT_Hc </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
</tr>
 
<tr class="row27">
 
<td class="col0"> 27 </td><td class="col1"> pIG15_1201 </td><td class="col2"> - </td><td class="col3"> Human Papilloma Virus, <br/>
 
L1/L2 </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
</tr>
 
<tr class="row28">
 
<td class="col0"> 28 </td><td class="col1"> pIG15_1301 </td><td class="col2"> - </td><td class="col3"> anti-putative dihydroxyacid dehydratase, <br/>
 
ScvF </td><td class="col4"> 6xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning + Expression </td>
 
 
</tr>
 
</tr>
 
<tr class="row29">
 
<tr class="row29">
 
<td class="col0"> 29 </td><td class="col1"> pRIG15_13 </td><td class="col2"> - </td><td class="col3"> anti-putative dihydroxyacid dehydratase, <br/>
 
<td class="col0"> 29 </td><td class="col1"> pRIG15_13 </td><td class="col2"> - </td><td class="col3"> anti-putative dihydroxyacid dehydratase, <br/>
 
ScvF </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
ScvF </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
</tr>
 
<tr class="row30">
 
<td class="col0"> 30 </td><td class="col1"> pIG15_1401 </td><td class="col2"> - </td><td class="col3"> anti-putative dimethyl sulfoxid reductase, <br/>
 
ScvF </td><td class="col4"> 6xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning + Expression </td>
 
</tr>
 
<tr class="row31">
 
<td class="col0"> 31 </td><td class="col1"> pIG15_1501 </td><td class="col2"> - </td><td class="col3"> <em>Salmonella typhimurium</em>, <br/>
 
putative dihydroxyacid dehydratase </td><td class="col4"> 6xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning + Expression </td>
 
 
</tr>
 
</tr>
 
<tr class="row32">
 
<tr class="row32">
<td class="col0"> 32 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pig15_1504.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pig15_1504.pdf">pIG15_1504</a> </td><td class="col2"> 10xHis </td><td class="col3"> <em>Salmonella typhimurium</em>, <br/>
+
<td class="col0"> 32 </td><td class="col1"> pIG15_1504 </td><td class="col2"> 10xHis </td><td class="col3"> <em>Salmonella typhimurium</em>, <br/>
 
putative dihydroxyacid dehydratase </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
putative dihydroxyacid dehydratase </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row33">
 
<tr class="row33">
<td class="col0"> 33 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1504.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1504.pdf">pET_1504</a> </td><td class="col2"> 6xHis </td><td class="col3"> <em>Salmonella typhimurium</em>, <br/>
+
<td class="col0"> 33 </td><td class="col1"> pET_1504 </td><td class="col2"> 6xHis </td><td class="col3"> <em>Salmonella typhimurium</em>, <br/>
 
putative dihydroxyacid dehydratase </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
putative dihydroxyacid dehydratase </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
</tr>
 
<tr class="row34">
 
<td class="col0"> 44 </td><td class="col1"> pIG15_1601 </td><td class="col2"> - </td><td class="col3"> <em>Salmonella typhimurium</em>, <br/>
 
putative dimethyl sulfoxid reductase </td><td class="col4"> 6xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning + Expression </td>
 
</tr>
 
<tr class="row35">
 
<td class="col0"> 45 </td><td class="col1"> pIG15_1701 </td><td class="col2"> - </td><td class="col3"> Human Immunodeficiency Virus, <br/>
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
</tr>
 
</tr>
 
<tr class="row36">
 
<tr class="row36">
<td class="col0"> 46 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pig15_1704.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pig15_1704.pdf">pIG15_1704</a> </td><td class="col2"> 10xHis </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
<td class="col0"> 46 </td><td class="col1"> pIG15_1704 </td><td class="col2"> 10xHis </td><td class="col3"> Human Immunodeficiency Virus, <br/>
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
+
</tr>
+
<tr class="row37">
+
<td class="col0"> 47 </td><td class="col1"> pIG15_1704_HA </td><td class="col2"> 10xHis_HA </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row38">
 
<tr class="row38">
<td class="col0"> 48 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1703.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1703.pdf">pET_1703</a> </td><td class="col2"> - </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
<td class="col0"> 48 </td><td class="col1"> pET_1703 </td><td class="col2"> - </td><td class="col3"> Human Immunodeficiency Virus, <br/>
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
+
</tr>
+
<tr class="row39">
+
<td class="col0"> 49 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1703-ha.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1703-ha.pdf">pET_1703_HA</a> </td><td class="col2"> HA </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row40">
 
<tr class="row40">
<td class="col0"> 50 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1704.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1704.pdf">pET_1704</a> </td><td class="col2"> 6xHis </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
<td class="col0"> 50 </td><td class="col1"> pET_1704 </td><td class="col2"> 6xHis </td><td class="col3"> Human Immunodeficiency Virus, <br/>
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
+
</tr>
+
<tr class="row41">
+
<td class="col0"> 51 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1704-ha.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1704-ha.pdf">pET_1704_HA</a> </td><td class="col2"> 6xHis_HA </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> Spy </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row42">
 
<tr class="row42">
<td class="col0"> 52 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:prig15_17.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:prig15_17.pdf">pRIG15_17</a> </td><td class="col2"> - </td><td class="col3"> Human Immunodeficiency Virus, <br/>
+
<td class="col0"> 52 </td><td class="col1"> pRIG15_17 </td><td class="col2"> - </td><td class="col3"> Human Immunodeficiency Virus, <br/>
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
immunodominant epitopes of the polyprotein tat/gag/pol/env </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
</tr>
 
<tr class="row43">
 
<td class="col0"> 53 </td><td class="col1"> pIG15_1801 </td><td class="col2"> - </td><td class="col3"> <em>Treponema pallidum</em>, <br/>
 
TpF1</td><td class="col4"> - </td><td class="col5"> Ampicillin </td><td class="col6"> Cloning </td>
 
 
</tr>
 
</tr>
 
<tr class="row44">
 
<tr class="row44">
<td class="col0"> 54 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pet_1803.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pet_1803.pdf">pET_1803</a> </td><td class="col2"> - </td><td class="col3"> <em>Treponema pallidum</em>, <br/>
+
<td class="col0"> 54 </td><td class="col1"> pET_1803 </td><td class="col2"> - </td><td class="col3"> <em>Treponema pallidum</em>, <br/>
 
TpF1 </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
TpF1 </td><td class="col4"> 10xHis </td><td class="col5"> Ampicillin </td><td class="col6"> Expression </td>
 
</tr>
 
</tr>
 
<tr class="row45">
 
<tr class="row45">
<td class="col0"> 55 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:prig15_18.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:prig15_18.pdf">pRIG15_18</a> </td><td class="col2"> - </td><td class="col3"> <em>Treponema pallidum</em>, <br/>
+
<td class="col0"> 55 </td><td class="col1"> pRIG15_18 </td><td class="col2"> - </td><td class="col3"> <em>Treponema pallidum</em>, <br/>
 
TpF1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
TpF1 </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> Submission </td>
 
</tr>
 
</tr>
 
<tr class="row46">
 
<tr class="row46">
<td class="col0"> 56 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pils1.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pils1.pdf">pILS_1</a> </td><td class="col2"> - </td><td class="col3"> GFP </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> InterLab Study </td>
+
<td class="col0"> 56 </td><td class="col1"> pILS_1 </td><td class="col2"> - </td><td class="col3"> GFP </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> InterLab Study </td>
 
</tr>
 
</tr>
 
<tr class="row47">
 
<tr class="row47">
<td class="col0"> 57 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pils2.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pils2.pdf">pILS_2</a></td><td class="col2"> - </td><td class="col3"> GFP </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> InterLab Study </td>
+
<td class="col0"> 57 </td><td class="col1"> pILS_2 </td><td class="col2"> - </td><td class="col3"> GFP </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> InterLab Study </td>
 
</tr>
 
</tr>
 
<tr class="row48">
 
<tr class="row48">
<td class="col0"> 58 </td><td class="col1"> <a href="/igem2015/lib/exe/fetch.php?media=labjournal:cloning:pils3.pdf" class="media mediafile mf_pdf" title="labjournal:cloning:pils3.pdf">pILS_3</a></td><td class="col2"> - </td><td class="col3"> GFP </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> InterLab Study </td>
+
<td class="col0"> 58 </td><td class="col1"> pILS_3 </td><td class="col2"> - </td><td class="col3"> GFP </td><td class="col4"> - </td><td class="col5"> Chloramphenicol </td><td class="col6"> InterLab Study </td>
 
</tr>
 
</tr>
 
<tr class="row49">
 
<tr class="row49">
Line 183: Line 123:
 
<tr class="row50">
 
<tr class="row50">
 
<td class="col0"> 60 </td><td class="col1"> pIG15_204 </td><td class="col2"> 10xHis </td><td class="col3"> EGFP </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
<td class="col0"> 60 </td><td class="col1"> pIG15_204 </td><td class="col2"> 10xHis </td><td class="col3"> EGFP </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
</tr>
 
<tr class="row51">
 
<td class="col0"> 61 </td><td class="col1"> pIG15_204_HA </td><td class="col2"> 10xHis_HA </td><td class="col3"> EGFP </td><td class="col4"> Spy </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
 
</tr>
 
</tr>
 
<tr class="row52">
 
<tr class="row52">
 
<td class="col0"> 62 </td><td class="col1"> pIG15_205 </td><td class="col2"> 10xHis </td><td class="col3"> EGFP </td><td class="col4"> Halo </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
<td class="col0"> 62 </td><td class="col1"> pIG15_205 </td><td class="col2"> 10xHis </td><td class="col3"> EGFP </td><td class="col4"> Halo </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
</tr>
 
<tr class="row53">
 
<td class="col0"> 63 </td><td class="col1"> pIG15_205_HA </td><td class="col2"> 10xHis_HA </td><td class="col3"> EGFP </td><td class="col4"> Halo </td><td class="col5"> Chloramphenicol </td><td class="col6"> Expression </td>
 
 
</tr>
 
</tr>
 
<tr class="row54">
 
<tr class="row54">

Revision as of 21:51, 17 September 2015

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Plasmids

No. Signature C-term. Tag Antigen N-term. Tag Resistence Purpose GenBank file
5 pET_603 - Rubella Virus,
glycoprotein E1
10xHis Ampicillin Expression pET_603.gb
6 pRIG15_6 - Rubella Virus,
glycoprotein E1
- Chloramphenicol Submission pRIG15_6.gb
8 pRIG15_7 - Human Herpes Virus,
glycoprotein E
- Chloramphenicol Submission pRIG15_7.gb
10 pIG15_804 10xHis Herpes simplex,
immunodominant epitope of glycoprotein G1
Spy Chloramphenicol Expression
11 pET_803 - Herpes simplex,
immunodominant epitope of glycoprotein G1
10xHis Ampicillin Expression pET_803.gb
12 pET_804 6xHis Herpes simplex,
immunodominant epitope of glycoprotein G1
Spy Ampicillin Expression pET_804.gb
13 - Herpes simplex,
immunodominant epitope of glycoprotein G1
- Chloramphenicol Submission
16 pET_1003 - Hepatits C,
core protein
10xHis Ampicillin Expression
17 pET_1004 6xHis Hepatits C,
core protein
Spy Ampicillin Expression
19 pIG15_1104 10xHis Clostridium tetani,
TeNT_Hc
Spy Chloramphenicol Expression
21 pIG15_1105 10xHis Clostridium tetani,
TeNT_Hc
Halo Chloramphenicol Expression
22 pET_1103 - Clostridium tetani,
TeNT_Hc
10xHis Ampicillin Expression
23 pET_1104 6xHis Clostridium tetani,
TeNT_Hc
Spy Ampicillin Expression
25 pET_1105 6xHis Clostridium tetani,
TeNT_Hc
Halo Ampicillin Expression
26 pRIG15_11 - Clostridium tetani,
TeNT_Hc
- Chloramphenicol Submission
29 pRIG15_13 - anti-putative dihydroxyacid dehydratase,
ScvF
- Chloramphenicol Submission
32 pIG15_1504 10xHis Salmonella typhimurium,
putative dihydroxyacid dehydratase
Spy Chloramphenicol Expression
33 pET_1504 6xHis Salmonella typhimurium,
putative dihydroxyacid dehydratase
Spy Ampicillin Expression
46 pIG15_1704 10xHis Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
Spy Chloramphenicol Expression
48 pET_1703 - Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
10xHis Ampicillin Expression
50 pET_1704 6xHis Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
Spy Ampicillin Expression
52 pRIG15_17 - Human Immunodeficiency Virus,
immunodominant epitopes of the polyprotein tat/gag/pol/env
- Chloramphenicol Submission
54 pET_1803 - Treponema pallidum,
TpF1
10xHis Ampicillin Expression
55 pRIG15_18 - Treponema pallidum,
TpF1
- Chloramphenicol Submission
56 pILS_1 - GFP - Chloramphenicol InterLab Study
57 pILS_2 - GFP - Chloramphenicol InterLab Study
58 pILS_3 - GFP - Chloramphenicol InterLab Study
59 pQE_EGFP_His6 - EGFP 6xHis Ampicillin Expression
60 pIG15_204 10xHis EGFP Spy Chloramphenicol Expression
62 pIG15_205 10xHis EGFP Halo Chloramphenicol Expression
64 pSB1C3 - - - Chloramphenicol Cloning
65 pSB1A3 - - - Ampicillin Cloning
66 Bba_I13504 - GFP - Chloramphenicol InterLab Study
67 Bba_K823005 - Anderson promoter 01 - Chloramphenicol InterLab Study
68 Bba_K823008 - Anderson promoter 06 - Chloramphenicol InterLab Study
69 Bba_K823013 - Anderson promoter 17 - Chloramphenicol InterLab Study
70 Bba_I20270 - const. Promoter + GFP - Chloramphenicol InterLab Study
71 Bba_R0040 - Tet-repressible promoter - Chloramphenicol InterLab Study
72 pTurboYFP(Evrogen) - TurboYFP - Neomycin Cloning
73 pIG15_104 10xHis TurboYFP Spy Chloramphenicol Expression
74 pIG15_105 10xHis TurboYFP Halo Chloramphenicol Expression
75 pIG15_001 - - - Chloramphenicol Cloning + Expression
76 pIG15_001-A - - - Ampicillin Cloning + Expression
77 pIG15_002 - - - Chloramphenicol Cloning + Expression
78 pIG15_002-A - - - Ampicillin Cloning + Expression
79 pIG15_403 - Dengue Virus,
non-structural protein 1
10xHis Chloramphenicol Cloning + Expression
80 pIG15_403-A - Dengue Virus,
non-structural protein 1
10xHis Ampicillin Cloning + Expression
81 pIG15_803 - Herpes simplex,
immunodominant epitope of glycoprotein G1
10xHis Chloramphenicol Cloning + Expression
82 pIG15_803-A - Herpes simplex,
immunodominant epitope of glycoprotein G1
10xHis Ampicillin Cloning + Expression
83 pRARE2 - - - Chloramphenicol Cell-free expession system