Difference between revisions of "Team:Freiburg/Project/Overview"
Line 757: | Line 757: | ||
<h1>DNA Engineering</h1> | <h1>DNA Engineering</h1> | ||
<p>Genetic fusion of different antigens and tags is a basic requirement of our project. In order to enable several people to work in parallel we designed a cloning strategy easy to follow and additionally easy to expand for further needs. | <p>Genetic fusion of different antigens and tags is a basic requirement of our project. In order to enable several people to work in parallel we designed a cloning strategy easy to follow and additionally easy to expand for further needs. | ||
− | Read more about combining different cloning methods to reduce efforts in | + | Read more about combining different cloning methods to reduce efforts in DNA Engineering and the design of an expression vector meeting iGEM requirements. |
</p> | </p> | ||
Revision as of 21:54, 17 September 2015
![](https://static.igem.org/mediawiki/2015/8/85/Freiburg_wiki_background_2_parted_left.png)
![](https://static.igem.org/mediawiki/2015/f/f4/Freiburg_wiki_background_2_parted_right.png)
write appropriate content for slider: should present main subpoints of project in short sentence and link to them
add pictures nto slider (ps1709)
The DiaCHIP - A Versatile Detection System
![DiaCHIP_Sabi](https://static.igem.org/mediawiki/2015/a/af/Freiburg_DiaCHIP_Sabi.png)
Project Motivation
Serological tests are a key element in modern medicine. Especially for detection and identification of infectious diseases, performing several blood tests is inevitable. Testing for more than one disease at a time or diagnosing a patient with diffuse symptoms usually requires more than one serological test. Every single test that is performed increases the time of waiting for a result as well as the bill. In case of dangerous infectious diseases every minute until the onset of the appropriate treatment is important for life. What if there was a possibility to combine all this testing in one single chip which is affordable for everyone?
Detecting Antigen-Antibody Interactions
The DiaCHIP is an innovative tool to screen for a broad range of antibodies present in serum. Antibodies serve as indicator for an immune response towards an infectious diseases or a successful vaccination. They also play an important role in the diagnosis of autoimmune diseases. Identifying diseases by detecting disease associated antibodies in a patient's serum is an established method in modern diagnostics.
Based on the very same principle, the DiaCHIP enables to simultaneously screen for multiple diseases at time, thereby reducing time and cost of a diagnosis. Especially the ability to differentiate between life threatening diseases and mild infections within a short time bears the potential to save lives.
The Concept
The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis of disease related antigens and a novel method for label-free detection - all this packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams.