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Revision as of 23:54, 17 September 2015

List of Parts

Registry Code
Name
Role
Description
Length (bp)
BBa_K1819003
RoxA
Protein coding
Rubber oxygenase A (Rox A) from Xanthomonas sp. strain 35Y- catalyzes oxidative C-C cleavage of poly(cis-1,4-isoprene)
1954
BBa_K1819001
Lcp
Role
Latex clearing protein (Lcp) from Streptomyces sp. strain K30 - catalyzes rubber cleavage at the cis double bonds
1138
BBa_K1819000
Linker-GFP
Reporter part
It is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site
750
BBa_K1819002
TAT signal
Signalling part
Twin-arginine translocation signal
147
BBa_K1819004
GFP_I13507
Composite part
RFP regulation through promoter tetR (part R0040), dependent of tetracycline
2499
BBa_K1819005
J23101 + linkerGFPTerm
Characterization circuit
Constitutive promotor from Anderson library (BBa_J23101) assembled to green fluorescent protein attached to linker
930
BBa_K1819006
J23106 + linkerGFPTerm
Characterization circuit
Constitutive promoter from Anderson library (BBa_J23106) assembled to green fluorescent protein attached to linker
930
BBa_K1819007
J23117 + linkerGFPTerm
Characterization circuit
Constitutive promotor from Anderson library (BBa_J23117) assembled to green fluorescent protein attached to linker
930
BBa_K1819008
Pl_I13507
Construction promoter test
Promoter test circuit to analyze the kill switch efficiency
2725
BBa_K1819009
pVeg + GFP_Term
Characterization circuit
This device express GFP under a strong constitutive Bacillus promoter control (pVeg) (BBa_K823003)
1123
BBa_S05291
RBS-OmpA
Construction intermediate
It is composed of a RBS preceding an outer membrane protein A (OmpA), that displays proteins on cell surface.
484
BBa_S05307
GFP_P0440
Construction intermediate
This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR
1568
BBa_S05308
Linker-GFP_Term
Construction intermediate
It is an intermediate construction of the export test circuit fused to a terminal region
887