Difference between revisions of "Team:UMaryland/HokSok"
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<p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;"><b>Hok/Sok Construct</b></p> | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;"><b>Hok/Sok Construct</b></p> | ||
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<p style="font-size:24px">Prior to experimentation, we had to insert the Hok-Sok construct into pSB1C3 in order to make it a BioBrick. We originally planned to PCR amplify the cassette out of the R1 plasmid of <i>E. coli</i>, but we were unable to find an suitable wild-type strain that was easily available. Instead, we turned to synthesizing the construct as a gBlock from IDT. As a 580 bp dsDNA fragment, it was suitable for addition via Gibson Assembly into pSB1C3. After subsequent transformation, miniprep, and confirmation sequencing, we had the first piece of our testing puzzle.</p> | <p style="font-size:24px">Prior to experimentation, we had to insert the Hok-Sok construct into pSB1C3 in order to make it a BioBrick. We originally planned to PCR amplify the cassette out of the R1 plasmid of <i>E. coli</i>, but we were unable to find an suitable wild-type strain that was easily available. Instead, we turned to synthesizing the construct as a gBlock from IDT. As a 580 bp dsDNA fragment, it was suitable for addition via Gibson Assembly into pSB1C3. After subsequent transformation, miniprep, and confirmation sequencing, we had the first piece of our testing puzzle.</p> | ||
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<p style="font-size:24px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Section Summary | <p style="font-size:24px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Section Summary | ||
<ul> | <ul> |
Revision as of 06:29, 18 September 2015