Difference between revisions of "Team:UMaryland/HokSok"

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<li>3. Do proximity effects occur between Hok-Sok and a neighboring expression construct? Alternatively, does the activity of Hok-Sok affect expression of a protein whose coding region is near the construct?</li>
 
<li>3. Do proximity effects occur between Hok-Sok and a neighboring expression construct? Alternatively, does the activity of Hok-Sok affect expression of a protein whose coding region is near the construct?</li>
 
</ul>
 
</ul>
<p align = "center><img src = "https://static.igem.org/mediawiki/2015/4/4a/UMDHappyCell.jpeg"></p>
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<p align = "center"><img src = "https://static.igem.org/mediawiki/2015/4/4a/UMDHappyCell.jpeg"></p>
 
<p style="font-size:24px">We hypothesized that Hok-Sok could maintain recombinant plasmids, as it does natural ones. We also hypothesized that Hok-Sok would have a slight negative effect on bacterial growth rate, in line with other alternative maintenance systems such as sRNBC (<a href = "http://parts.igem.org/Part:BBa_K817015">K817015</a>), as well as the amount of protein expression due to competing parallel promoters. In order to answer these questions, we set up a variety of testing procedures, as shown below.</p>  
 
<p style="font-size:24px">We hypothesized that Hok-Sok could maintain recombinant plasmids, as it does natural ones. We also hypothesized that Hok-Sok would have a slight negative effect on bacterial growth rate, in line with other alternative maintenance systems such as sRNBC (<a href = "http://parts.igem.org/Part:BBa_K817015">K817015</a>), as well as the amount of protein expression due to competing parallel promoters. In order to answer these questions, we set up a variety of testing procedures, as shown below.</p>  
 
<p style="font-size:24px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Section Summary</p>
 
<p style="font-size:24px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Section Summary</p>

Revision as of 06:48, 18 September 2015