Difference between revisions of "Team:UMaryland/Results"

Revision as of 07:30, 18 September 2015

Plating Tests

Negative Control (No Pressure)

Figure 1. Negative control was K1783002 (constitutive unstable RFP) grown in media without chloramphenicol. After 2-3 days, plates demonstrate fewer than 10 colonies per plate, suggesting that antibiotic resistance is now only minimally present in the culture.

Chloramphenicol Pressure Only

Figure 2. K1783002 (constitutive unstable RFP) grown in media with chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.

Chlor + Hok-Sok Pressure

Figure 3. K1783003 (constitutive unstable RFP under Hok-Sok regulation) grown in media with chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.

Hok-Sok Pressure Only

Figure 4. K1783003 (constitutive unstable RFP under Hok-Sok regulation) grown in media without chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.

Growth Curve

Figure 5. Growth curves of parallel cultures grown with and without chloramphenicol. The presence of Hok-Sok on the inserted plasmid does not appear to have a major effect on the bacterial growth rate. This is in line with our qualitative observations, where we do not observe any major difference in cell density during plating or fluorescence studies.

Fluorescence Studies

Analysis of Fluorescence Loss

Over time, colonies appear to stop producing RFP.

This effect occurs even when the culture is under chloramphenicol pressure.

This effect occurs much more slowly when the Hok-Sok cassette is adjacent to the RFP construct.

With or without added chloramphenicol, Hok-Sok appears to be able to maintain a relatively constant level of fluorescence.

Sequence Analysis

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 <p><img src= "https://static.igem.org/mediawiki/2015/thumb/9/92/UMaryland_Group_E_090615.JPG/800px-UMaryland_Group_E_090615.JPG"height="333px" width="500px"></p>
 <p style = "font-size:18px">With or without added chloramphenicol, Hok-Sok appears to be able to maintain a relatively constant level of fluorescence.</p>
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