Difference between revisions of "Team:UMaryland/Notebook"
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<p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | <p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | ||
<b>Week 2</b></a> | <b>Week 2</b></a> | ||
− | <p style="font-size:24px;text-align:left;"> | + | <p style="font-size:24px;text-align:left;text-decoration: underline;"> |
Miraculin: | Miraculin: | ||
− | < | + | <ul class="a"> |
− | The pBAD +miraculin construct was moved back into psb1C3 backbone and sent for sequencing | + | <li>The pBAD +miraculin construct was moved back into psb1C3 backbone and sent for sequencing</li> |
− | < | + | </ul> |
+ | <p style="font-size:24px;text-align:left;text-decoration: underline;"> | ||
Designing gBlocks: | Designing gBlocks: | ||
− | < | + | <ul class="a"> |
− | Designed gblocks for epsilon-cyclase (from arabidopsis), pyocin and Hok/Sok | + | <li>Designed gblocks for epsilon-cyclase (from arabidopsis), pyocin and Hok/Sok</li> |
+ | </ul> | ||
<br> | <br> | ||
<br> | <br> | ||
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<p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | <p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | ||
<b>Week3</b></a> | <b>Week3</b></a> | ||
− | <p style="font-size:24px;text-align:left;"> | + | <p style="font-size:24px;text-align:left;text-decoration: underline;"> |
Miraculin: | Miraculin: | ||
<br> | <br> | ||
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<p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | <p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | ||
<b>Week 4</b></a> | <b>Week 4</b></a> | ||
− | <p style="font-size:24px;text-align:left;"> | + | <p style="font-size:24px;text-align:left;text-decoration: underline;"> |
Miraculin: | Miraculin: | ||
<br> | <br> | ||
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<p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | <p style="font-size:32px;text-align:left;font-family:Verdana, Geneva, sans-serif;"> | ||
<b>Week 5</b></a> | <b>Week 5</b></a> | ||
− | <p style="font-size:24px;text-align:left;"> | + | <p style="font-size:24px;text-align:left;text-decoration: underline;"> |
Miraculin: | Miraculin: | ||
<br> | <br> | ||
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SDS page showed a band at roughly 25 kDa | SDS page showed a band at roughly 25 kDa | ||
<br> | <br> | ||
− | Hok/Sok | + | <p style="font-size:24px;text-align:left;text-decoration: underline;"> |
+ | Hok/Sok: | ||
<br> | <br> | ||
inserted Hok/Sok gblock into PSB1C3 backbone using Gibson assembly | inserted Hok/Sok gblock into PSB1C3 backbone using Gibson assembly | ||
Line 367: | Line 370: | ||
construct sent for sequencing | construct sent for sequencing | ||
<br> | <br> | ||
− | Interlab Study | + | <p style="font-size:24px;text-align:left;text-decoration: underline;"> |
+ | Interlab Study: | ||
<br> | <br> | ||
transformed parts into dh5a: 3 Anderson promoters, GFP, and RFP | transformed parts into dh5a: 3 Anderson promoters, GFP, and RFP |
Revision as of 09:16, 18 September 2015