Difference between revisions of "Team:Stanford-Brown/Parts"

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       <a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank">
 
       <a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank">
 
         <h4>Biobrick : BBa_K1692028</h4>
 
         <h4>Biobrick : BBa_K1692028</h4>
         <p><b>cotz-aeBlue-CipA</b> the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. </p>
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         <p><b>cotz-aeBlue-CipA</b> is a fusion protein consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. </p>
 
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Revision as of 22:13, 18 September 2015

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