<div class="col-sm-6">

      <a href="http://parts.igem.org/Part:BBa_K1692028" class="btn" id="be1" target="_blank">

        <h4>Biobrick: BBa_K1692028</h4>

        <p><b>cotz-aeBlue-CipA</b> the first step involved cloning a Bacillus construct in Escherichia coli of a fusion protein sequencing consisting of a spore coat protein, cotZ (building off work done on Sporobeads by the LMU Munich 2012 iGEM team), and a cellulose binding domain (CIPA). Additionally, we decided to add aeBlue, a chromogenic protein, between cotZ and CIPA to be able to see with the naked eye whether Bacillus is in a vegetative or a spore state. </p>

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<h2>Polystyrene Synthesis BioBricks</h2>

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      <a href="http://parts.igem.org/Part:BBa_K1692007" class="btn" id="be1" target="_blank">

        <h4>Biobrick: BBa_K1692007</h4>

        <p><b>UbiX with T7 promoter and FLAG tag</b> UbiX is a flavin prenyltransferase that normally plays a role in ubiquinone biosynthesis in <i>E. coli</i>. UbiX transfers a prenyl group from dimethylallyl monophosphate (DMAP) to flavin mononucleotide (FMN), thereby creating a cofactor that happens to be essential to the functionality of FDC. Our genetic construct includes the FDC gene, a T7 inducible promoter, a ribosome binding site, and a FLAG-tag peptide sequence for easy and efficient protein purification. We have sequenced our construct and verified that all these components are indeed present.  </p>

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      <a href="http://parts.igem.org/Part:BBa_K1692008" class="btn" id="be1" target="_blank">

        <h4>Biobrick: BBa_K1692008</h4>

        <p><b>Styrene Synthesis Operon</b> Combo Plasmid containing a fusion protein of all three enzymes required to synthesise styrene from the starting organic reagent L-Phenylalanine.</p>

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      <a href="http://parts.igem.org/Part:BBa_K1692000" class="btn" id="be1" target="_blank">

        <h4>Biobrick: BBa_K1692000</h4>

        <p><b>Ferulic acid decarboxylase</b> Ferulic Acid Decarboxylase is used to synthesize styrene from trans-cinnamic acid </p>

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  <h2>P(3HB) Synthesis BioBricks</h2>

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      <a href="http://parts.igem.org/Part:BBa_K1692020" class="btn" id="be1" target="_blank">

        <h4>Biobrick: BBa_K1692020</h4>

        <p><b>S. aureus type II PanK (CoaA)</b> Type II pantothenate kinase from <i>Staphylococcus aureus</i>, not sensitive to feedback inhibition from CoA. </p>

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      <a href="http://parts.igem.org/Part:BBa_K1692034" class="btn" id="be1" target="_blank">

        <h4>Biobrick: BBa_K1692034</h4>

        <p><b>Pank + hybrid phaCAB + lysis</b> Construct that was cloned to have P(3HB) producing <i>E. coli</i> cells lyse when induced with Arabinose. This would greatly facilitate P(3HB) extraction. We are currently still testing and characterizing this part.  </p>

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      <a href="http://parts.igem.org/Part:BBa_K1692023" class="btn" id="be2" target="_blank">

        <h4>Biobrick: BBa_K1692023</h4>

        <p><b>Ptet + Luxl</b>Construct that was cloned to have P(3HB) producing <i>E. coli</i> cells lyse after reaching a certain population density using a quorum sensing promoter. This would greatly facilitate P(3HB) extraction. We are currently still testing and characterizing this part. </p>

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      <a href="http://parts.igem.org/Part:BBa_K1692024" class="btn" id="be2" target="_blank">

        <h4>Biobrick: BBa_K1692024</h4>

        <p><b>Autolysis</b>Construct that was cloned to have P(3HB) producing <i>E. coli</i> cells lyse after reaching a certain population density using a quorum sensing promoter. The difference between this brick BBa_K1692023 is that the basic parts composing this gene are in a different order, which is part of our testing to optimize this system. This would greatly facilitate P(3HB) extraction. We are currently still testing and characterizing this part. </p>

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  <h2>Cellulose Sheets BioBricks</h2>

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  <h2>CRATER BioBricks</h2>

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      <a href="http://parts.igem.org/Part:BBa_K1692033" class="btn" id="be1" target="_blank">

        <h4>Biobrick : BBa_K1692033</h4>

        <p><b>RFP gDNA</b> This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). When used in conjuction with the CRISPR/Cas system, this gRNA will bind to and digest the RFP plasmid. </p>

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      <a href="http://parts.igem.org/Part:BBa_K1692030" class="btn" id="be2" target="_blank">

        <h4>Biobrick: BBa_K1692030</h4>

        <p><b>amilGFP yellow chromoprotein, RBS and promoter</b>This plasmid contains the amilGFP yellow chromoprotein gene from part BBa_K1033931 with the RBS and promoter from part BBa_K608002.</p>

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      <a href="http://parts.igem.org/Part:BBa_K1692031" class="btn" id="be2" target="_blank">

        <h4>Biobrick: BBa_K1692031</h4>

        <p><b>meffBlue blue chromoprotein with RBS and promoter</b>This plasmid contains the meffBlue blue chromoprotein gene found in the BBa_K1033902 part, with the RBS and promoter from part BBa_K608002.</p>

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      <a href="http://parts.igem.org/Part:BBa_K1692032" class="btn" id="be2" target="_blank">

        <h4>Biobrick: BBa_K1692032</h4>

        <p><b>amilCP blue chromoprotein with RBS and promoter</b>This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002.</p>

      </a>

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