Difference between revisions of "Team:British Columbia/Basic Part"

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Revision as of 03:35, 19 September 2015

UBC iGEM 2015

 

Basic Parts

 

Cch2 (BBa_K1813000)

.

Codon optimized coding sequence of cch2, an enzyme from the ''Bradyrhizobiaceae'' bacterium SG-6C, for E.Coli. This part was used to make our part BBa_K1813001 .

Originally cloned and characterized by Shettigar et al. from Bradyrhizobiaceae bacterial strain SG 6C [1].

The enzyme coded for by cch2 converts 6-chloronicotinic acid (6-CNA) to 6-Hydronicotinic acid (6-HNA).

1] Shettigar M, Pearce S, Pandey R, Khan F, Dorrian SJ, et al. (2012) Cloning of a Novel 6-Chloronicotinic Acid Chlorohydrolase from the Newly Isolated 6-Chloronicotinic Acid Mineralizing Bradyrhizobiaceae Strain SG-6C. PLoS ONE 7(11): e51162. doi: 10.1371/journal.pone.0051162

here!