Difference between revisions of "Team:Edinburgh/Composite Part"

Line 116: Line 116:
 
         </div>
 
         </div>
 
       </header>
 
       </header>
<section id="about">
 
    <div class="container">
 
            <div class="row">
 
                <div class="col-lg-12 text-center">
 
                    <h2 class="section-heading">Heroin Esterase</h2>                   
 
                </div>
 
            </div>
 
  </div>
 
</section>
 
  
<div class="container">
 
 
    <div id="accordion" class="panel-group">
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne">1. What is HTML?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseOne" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. <a href="http://www.tutorialrepublic.com/html-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseTwo">2. What is Bootstrap?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseTwo" class="panel-collapse collapse in">
 
 
                <div class="panel-body">
 
 
                    <p>Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. <a href="http://www.tutorialrepublic.com/twitter-bootstrap-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">3. What is CSS?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseThree" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. <a href="http://www.tutorialrepublic.com/css-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
    </div>
 
</div>
 
 
 
<section id="about">
 
    <div class="container">
 
            <div class="row">
 
                <div class="col-lg-12 text-center">
 
                    <h2 class="section-heading">Morphine-6-Dehydrogenase</h2>                   
 
                </div>
 
            </div>
 
  </div>
 
</section>
 
 
<div class="container">
 
 
    <div id="accordion" class="panel-group">
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne">1. What is HTML?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseOne" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. <a href="http://www.tutorialrepublic.com/html-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseTwo">2. What is Bootstrap?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseTwo" class="panel-collapse collapse in">
 
 
                <div class="panel-body">
 
 
                    <p>Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. <a href="http://www.tutorialrepublic.com/twitter-bootstrap-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">3. What is CSS?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseThree" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. <a href="http://www.tutorialrepublic.com/css-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
    </div>
 
</div>
 
 
<section id="about">
 
    <div class="container">
 
            <div class="row">
 
                <div class="col-lg-12 text-center">
 
                    <h2 class="section-heading">Monoamine Oxidase A</h2>                   
 
                </div>
 
            </div>
 
  </div>
 
</section>
 
 
<div class="container">
 
 
    <div id="accordion" class="panel-group">
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne">1. What is HTML?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseOne" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. <a href="http://www.tutorialrepublic.com/html-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseTwo">2. What is Bootstrap?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseTwo" class="panel-collapse collapse in">
 
 
                <div class="panel-body">
 
 
                    <p>Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. <a href="http://www.tutorialrepublic.com/twitter-bootstrap-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">3. What is CSS?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseThree" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. <a href="http://www.tutorialrepublic.com/css-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
    </div>
 
</div>
 
 
<section id="about">
 
    <div class="container">
 
            <div class="row">
 
                <div class="col-lg-12 text-center">
 
                    <h2 class="section-heading">Laccase</h2>                   
 
                </div>
 
            </div>
 
  </div>
 
</section>
 
 
<div class="container">
 
 
    <div id="accordion" class="panel-group">
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne">1. What is HTML?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseOne" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. <a href="http://www.tutorialrepublic.com/html-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseTwo">2. What is Bootstrap?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseTwo" class="panel-collapse collapse in">
 
 
                <div class="panel-body">
 
 
                    <p>Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. <a href="http://www.tutorialrepublic.com/twitter-bootstrap-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
        <div class="panel panel-default">
 
 
            <div class="panel-heading">
 
 
                <h4 class="panel-title">
 
 
                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">3. What is CSS?</a>
 
 
                </h4>
 
 
            </div>
 
 
            <div id="collapseThree" class="panel-collapse collapse">
 
 
                <div class="panel-body">
 
 
                    <p>CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. <a href="http://www.tutorialrepublic.com/css-tutorial/" target="_blank">Learn more.</a></p>
 
 
                </div>
 
 
            </div>
 
 
        </div>
 
 
    </div>
 
</div>
 
  
 
<section id="about">
 
<section id="about">

Revision as of 03:54, 19 September 2015

mRFP

mRFP (BBa_K1615089) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the mRFP gene to express mRFP when induced with IPTG.

mRFP (BBa_K1615089) fused to the C terminal of CBDcenA + C-terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator.

mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.



We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.

We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.

mRFP (BBa_K1615089) fused to the C terminal of dCBD + N terminal linker (BBa_K1321340). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.


To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator.