Difference between revisions of "Team:British Columbia/Notebook/Protocols"
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<p>To amplify an insert for cloning, using high-fidelity phusion polymerase.</p> | <p>To amplify an insert for cloning, using high-fidelity phusion polymerase.</p> | ||
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<p>Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.</p> | <p>Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.</p> | ||
| − | <p><a class="btn btn-default" href=" | + | <p><a class="btn btn-default" href="https://2015.igem.org/wiki/index.php?title=Team:British_Columbia/Notebook/Protocols/ColonyPCR" role="button">View details »</a></p> |
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Revision as of 00:10, 1 April 2015
Protocols: Use the categories on the left to find the protocol you need:
To amplify an insert for cloning, using high-fidelity phusion polymerase.
Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.
Before primers can be used for PCR they need to be resuspended and diluted.
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hirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae.Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae. Thirdamuno, ipsum dolor sit amet, consectetur adipiscing elit. Duis elementum auctor accumsan. Duis pharetra varius quam sit amet vulputate. Quisque mauris augue, molestie tincidunt condimentum vitae.