Difference between revisions of "Team:Carnegie Mellon/Interlab"

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As described on the iGEM website, <font color = orange>"</font><font color = #40e6ee><i>The goal of the InterLab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world.</i></font><font color = orange>"</font> Part of the purpose of the study is to quantify the variability of data collection across labs and to test the reproducibility of the promoters used.
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<center>As described on the iGEM website, <font color = orange>"</font><font color = #40e6ee><i>The goal of the InterLab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world.</i></font><font color = orange>"</font> Part of the purpose of the study is to quantify the variability of data collection across labs and to test the reproducibility of the promoters used.</center>
 
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Three plasmids were constructed using parts given to us from the iGEM registry. The first contained a high expressing constitutive J23101 promoter with GFP, while the second one contained a medium expressing constitutive J23106 promoter with GFP, and the third contained a very low-expressing constitutive J23117 promoter with GFP. Diagrams of the three plasmids are shown below.
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Revision as of 17:51, 5 August 2015

Under Construction.

Interlabz.

As described on the iGEM website, "The goal of the InterLab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around the world." Part of the purpose of the study is to quantify the variability of data collection across labs and to test the reproducibility of the promoters used.


Study Description
Three plasmids were constructed using parts given to us from the iGEM registry. The first contained a high expressing constitutive J23101 promoter with GFP, while the second one contained a medium expressing constitutive J23106 promoter with GFP, and the third contained a very low-expressing constitutive J23117 promoter with GFP. Diagrams of the three plasmids are shown below.