Difference between revisions of "Team:Edinburgh/Composite Part"
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− | {{ | + | {{Edinburgh_practices}} |
<html> | <html> | ||
− | |||
+ | <head> | ||
+ | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css"> | ||
+ | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/js/bootstrap.min.js"></script> | ||
+ | </head> | ||
− | <div class=" | + | <body> |
− | < | + | |
− | < | + | <!-- menu --> |
+ | <div class="navbar-wrapper"> | ||
+ | <div class="container"> | ||
+ | <nav class="navbar transparent navbar-default navbar-fixed-top"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar"> | ||
+ | <span class="sr-only">Toggle navigation</span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | </div> | ||
+ | <div id="navbar" class="navbar-collapse collapse"> | ||
+ | <ul class="nav navbar-nav"> | ||
+ | <li class="active"> | ||
+ | <a href="https://2015.igem.org/Team:Edinburgh">Home</a></li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-expanded="false">Project<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu" role="menu"> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Description">Description</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Experiments">Experiments</a> </li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Project/Protocols">Protocols</a> </li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Results">Results</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Design">Design</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-expanded="false">Parts<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu" role="menu"> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Parts">Team Parts</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Basic_Part">Basic Parts</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Composite_Part">Composite Parts</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Part_Collection">Part Collection</a> </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Practices">Policy and Practices</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Safety">Safety</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/Modeling">Modeling</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Edinburgh/InterLab">InterLab</a></li> | ||
+ | <li><a href="software.html">Software</a></li> | ||
+ | <li><a href="entrepreneurship.html">Entrepreneurship</a></li> | ||
+ | <li><a href="collaborations.html">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- End of menu --> | ||
+ | |||
+ | <header class="intro"> | ||
+ | <div class="intro-body"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-12"> | ||
+ | <h1 class="brand-heading">Basic Parts</h1> | ||
+ | <p class="intro-text"> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </header> | ||
+ | |||
+ | |||
+ | <div class="container"> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading"> | ||
+ | <h3 class="panel-title">Panel title</h3> | ||
+ | </div> | ||
</div> | </div> | ||
+ | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingOne"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne"> | ||
+ | 1% Agarose Gel | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p style="color: black;"> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>1g Agarose | ||
+ | <li>100ml 1X TAE buffer | ||
+ | <li>5µl GelRed stain | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p class="text-muted"> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Mix the agarose with the 1X TAE buffer in a flask. | ||
+ | <li>2. Heat the mixture until all the agarose is dissolved. | ||
+ | <li>3. Swirl the flask under cold running water to cool the mixture. | ||
+ | <li> 4. Add the gel stain. | ||
+ | <li>5. Pour into an assembled gel tray and let it cool. | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingTwo"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo"> | ||
+ | Agarose Gel Electrophoresis | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>1% Agarose | ||
+ | <li>1X TAE buffer | ||
+ | <li>5X loading dye | ||
+ | <li>DNA ladder | ||
+ | <li>DNA samples | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Place gel tray into the electrophoresis apparatus. | ||
+ | <li>2. Pour 1X TAE so that the gel is covered by buffer. | ||
+ | <li>3. Prepare the samples by adding the appropriate amount of loading dye. | ||
+ | <li>4. Load samples and DNA ladder into wells on the gel. | ||
+ | <li>5. Run the gel at roughly 100V for around an hour | ||
− | <p> | + | </uL> |
− | + | </p> | |
− | </p> | + | </div> |
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingThree"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree"> | ||
+ | Culture | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>10ml Luria Broth (LB) | ||
+ | <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin) | ||
+ | <li>Loop (for picking colony) | ||
+ | <li>Ethanol | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Pour 10ml of LB into a 50ml Falcon tube. | ||
+ | <li>2. Pipette 10µl of antibiotic into the broth. | ||
+ | <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube. | ||
+ | <li>4. Incubate at 37°C overnight in a shaking incubator. | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
− | <p> | + | <div class="panel panel-default"> |
+ | <div class="panel-heading"> | ||
+ | <h3 class="panel-title">Panel title</h3> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingOne"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne"> | ||
+ | 1% Agarose Gel | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p style="color: black;"> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>1g Agarose | ||
+ | <li>100ml 1X TAE buffer | ||
+ | <li>5µl GelRed stain | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p class="text-muted"> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Mix the agarose with the 1X TAE buffer in a flask. | ||
+ | <li>2. Heat the mixture until all the agarose is dissolved. | ||
+ | <li>3. Swirl the flask under cold running water to cool the mixture. | ||
+ | <li> 4. Add the gel stain. | ||
+ | <li>5. Pour into an assembled gel tray and let it cool. | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingTwo"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo"> | ||
+ | Agarose Gel Electrophoresis | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>1% Agarose | ||
+ | <li>1X TAE buffer | ||
+ | <li>5X loading dye | ||
+ | <li>DNA ladder | ||
+ | <li>DNA samples | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Place gel tray into the electrophoresis apparatus. | ||
+ | <li>2. Pour 1X TAE so that the gel is covered by buffer. | ||
+ | <li>3. Prepare the samples by adding the appropriate amount of loading dye. | ||
+ | <li>4. Load samples and DNA ladder into wells on the gel. | ||
+ | <li>5. Run the gel at roughly 100V for around an hour | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingThree"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree"> | ||
+ | Culture | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>10ml Luria Broth (LB) | ||
+ | <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin) | ||
+ | <li>Loop (for picking colony) | ||
+ | <li>Ethanol | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Pour 10ml of LB into a 50ml Falcon tube. | ||
+ | <li>2. Pipette 10µl of antibiotic into the broth. | ||
+ | <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube. | ||
+ | <li>4. Incubate at 37°C overnight in a shaking incubator. | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingFour"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour"> | ||
+ | Gel Purification using QIAquick Gel Extraction Kit | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading"> | ||
+ | <h3 class="panel-title">Panel title</h3> | ||
+ | </div> | ||
</div> | </div> | ||
+ | <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true"> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingOne"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="false" aria-controls="collapseOne"> | ||
+ | 1% Agarose Gel | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseOne" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingOne"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p style="color: black;"> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>1g Agarose | ||
+ | <li>100ml 1X TAE buffer | ||
+ | <li>5µl GelRed stain | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p class="text-muted"> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Mix the agarose with the 1X TAE buffer in a flask. | ||
+ | <li>2. Heat the mixture until all the agarose is dissolved. | ||
+ | <li>3. Swirl the flask under cold running water to cool the mixture. | ||
+ | <li> 4. Add the gel stain. | ||
+ | <li>5. Pour into an assembled gel tray and let it cool. | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingTwo"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo"> | ||
+ | Agarose Gel Electrophoresis | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseTwo" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingTwo"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>1% Agarose | ||
+ | <li>1X TAE buffer | ||
+ | <li>5X loading dye | ||
+ | <li>DNA ladder | ||
+ | <li>DNA samples | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Place gel tray into the electrophoresis apparatus. | ||
+ | <li>2. Pour 1X TAE so that the gel is covered by buffer. | ||
+ | <li>3. Prepare the samples by adding the appropriate amount of loading dye. | ||
+ | <li>4. Load samples and DNA ladder into wells on the gel. | ||
+ | <li>5. Run the gel at roughly 100V for around an hour | ||
+ | |||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingThree"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="false" aria-controls="collapseThree"> | ||
+ | Culture | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | <div id="collapseThree" class="panel-collapse collapse" role="tabpanel" aria-labelledby="headingThree"> | ||
+ | <div class="panel-body"> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Materials</h2> | ||
+ | <ul> | ||
+ | <li>10ml Luria Broth (LB) | ||
+ | <li>10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin) | ||
+ | <li>Loop (for picking colony) | ||
+ | <li>Ethanol | ||
+ | </ul> | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <p> | ||
+ | <h2>Procedure</h2> | ||
+ | <ul> | ||
+ | <li>1. Pour 10ml of LB into a 50ml Falcon tube. | ||
+ | <li>2. Pipette 10µl of antibiotic into the broth. | ||
+ | <li>3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube. | ||
+ | <li>4. Incubate at 37°C overnight in a shaking incubator. | ||
+ | </uL> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="panel panel-default"> | ||
+ | <div class="panel-heading" role="tab" id="headingFour"> | ||
+ | <h4 class="panel-title"> | ||
+ | <a class="collapsed" role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="false" aria-controls="collapseFour"> | ||
+ | Gel Purification using QIAquick Gel Extraction Kit | ||
+ | </a> | ||
+ | </h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <footer> | ||
+ | <p class="pull-right"><a href="#">Back to top</a></p> | ||
+ | <p>© 2015 EdiGEM · <a href="#">Privacy</a> · <a href="#">Terms</a></p> | ||
+ | </footer> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 10:42, 1 September 2015
Basic Parts
Panel title
Materials
- 1g Agarose
- 100ml 1X TAE buffer
- 5µl GelRed stain
Procedure
- 1. Mix the agarose with the 1X TAE buffer in a flask.
- 2. Heat the mixture until all the agarose is dissolved.
- 3. Swirl the flask under cold running water to cool the mixture.
- 4. Add the gel stain.
- 5. Pour into an assembled gel tray and let it cool.
Materials
- 1% Agarose
- 1X TAE buffer
- 5X loading dye
- DNA ladder
- DNA samples
Procedure
- 1. Place gel tray into the electrophoresis apparatus.
- 2. Pour 1X TAE so that the gel is covered by buffer.
- 3. Prepare the samples by adding the appropriate amount of loading dye.
- 4. Load samples and DNA ladder into wells on the gel.
- 5. Run the gel at roughly 100V for around an hour
Materials
- 10ml Luria Broth (LB)
- 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
- Loop (for picking colony)
- Ethanol
Procedure
- 1. Pour 10ml of LB into a 50ml Falcon tube.
- 2. Pipette 10µl of antibiotic into the broth.
- 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
- 4. Incubate at 37°C overnight in a shaking incubator.
Panel title
Materials
- 1g Agarose
- 100ml 1X TAE buffer
- 5µl GelRed stain
Procedure
- 1. Mix the agarose with the 1X TAE buffer in a flask.
- 2. Heat the mixture until all the agarose is dissolved.
- 3. Swirl the flask under cold running water to cool the mixture.
- 4. Add the gel stain.
- 5. Pour into an assembled gel tray and let it cool.
Materials
- 1% Agarose
- 1X TAE buffer
- 5X loading dye
- DNA ladder
- DNA samples
Procedure
- 1. Place gel tray into the electrophoresis apparatus.
- 2. Pour 1X TAE so that the gel is covered by buffer.
- 3. Prepare the samples by adding the appropriate amount of loading dye.
- 4. Load samples and DNA ladder into wells on the gel.
- 5. Run the gel at roughly 100V for around an hour
Materials
- 10ml Luria Broth (LB)
- 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
- Loop (for picking colony)
- Ethanol
Procedure
- 1. Pour 10ml of LB into a 50ml Falcon tube.
- 2. Pipette 10µl of antibiotic into the broth.
- 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
- 4. Incubate at 37°C overnight in a shaking incubator.
Panel title
Materials
- 1g Agarose
- 100ml 1X TAE buffer
- 5µl GelRed stain
Procedure
- 1. Mix the agarose with the 1X TAE buffer in a flask.
- 2. Heat the mixture until all the agarose is dissolved.
- 3. Swirl the flask under cold running water to cool the mixture.
- 4. Add the gel stain.
- 5. Pour into an assembled gel tray and let it cool.
Materials
- 1% Agarose
- 1X TAE buffer
- 5X loading dye
- DNA ladder
- DNA samples
Procedure
- 1. Place gel tray into the electrophoresis apparatus.
- 2. Pour 1X TAE so that the gel is covered by buffer.
- 3. Prepare the samples by adding the appropriate amount of loading dye.
- 4. Load samples and DNA ladder into wells on the gel.
- 5. Run the gel at roughly 100V for around an hour
Materials
- 10ml Luria Broth (LB)
- 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
- Loop (for picking colony)
- Ethanol
Procedure
- 1. Pour 10ml of LB into a 50ml Falcon tube.
- 2. Pipette 10µl of antibiotic into the broth.
- 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
- 4. Incubate at 37°C overnight in a shaking incubator.