Difference between revisions of "Team:Edinburgh/Composite Part"
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Revision as of 10:44, 1 September 2015
Composite Parts
Heroin Esterase
Materials
- 1g Agarose
- 100ml 1X TAE buffer
- 5µl GelRed stain
Procedure
- 1. Mix the agarose with the 1X TAE buffer in a flask.
- 2. Heat the mixture until all the agarose is dissolved.
- 3. Swirl the flask under cold running water to cool the mixture.
- 4. Add the gel stain.
- 5. Pour into an assembled gel tray and let it cool.
Materials
- 1% Agarose
- 1X TAE buffer
- 5X loading dye
- DNA ladder
- DNA samples
Procedure
- 1. Place gel tray into the electrophoresis apparatus.
- 2. Pour 1X TAE so that the gel is covered by buffer.
- 3. Prepare the samples by adding the appropriate amount of loading dye.
- 4. Load samples and DNA ladder into wells on the gel.
- 5. Run the gel at roughly 100V for around an hour
Materials
- 10ml Luria Broth (LB)
- 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
- Loop (for picking colony)
- Ethanol
Procedure
- 1. Pour 10ml of LB into a 50ml Falcon tube.
- 2. Pipette 10µl of antibiotic into the broth.
- 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
- 4. Incubate at 37°C overnight in a shaking incubator.
Panel title
Materials
- 1g Agarose
- 100ml 1X TAE buffer
- 5µl GelRed stain
Procedure
- 1. Mix the agarose with the 1X TAE buffer in a flask.
- 2. Heat the mixture until all the agarose is dissolved.
- 3. Swirl the flask under cold running water to cool the mixture.
- 4. Add the gel stain.
- 5. Pour into an assembled gel tray and let it cool.
Materials
- 1% Agarose
- 1X TAE buffer
- 5X loading dye
- DNA ladder
- DNA samples
Procedure
- 1. Place gel tray into the electrophoresis apparatus.
- 2. Pour 1X TAE so that the gel is covered by buffer.
- 3. Prepare the samples by adding the appropriate amount of loading dye.
- 4. Load samples and DNA ladder into wells on the gel.
- 5. Run the gel at roughly 100V for around an hour
Materials
- 10ml Luria Broth (LB)
- 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
- Loop (for picking colony)
- Ethanol
Procedure
- 1. Pour 10ml of LB into a 50ml Falcon tube.
- 2. Pipette 10µl of antibiotic into the broth.
- 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
- 4. Incubate at 37°C overnight in a shaking incubator.
Panel title
Materials
- 1g Agarose
- 100ml 1X TAE buffer
- 5µl GelRed stain
Procedure
- 1. Mix the agarose with the 1X TAE buffer in a flask.
- 2. Heat the mixture until all the agarose is dissolved.
- 3. Swirl the flask under cold running water to cool the mixture.
- 4. Add the gel stain.
- 5. Pour into an assembled gel tray and let it cool.
Materials
- 1% Agarose
- 1X TAE buffer
- 5X loading dye
- DNA ladder
- DNA samples
Procedure
- 1. Place gel tray into the electrophoresis apparatus.
- 2. Pour 1X TAE so that the gel is covered by buffer.
- 3. Prepare the samples by adding the appropriate amount of loading dye.
- 4. Load samples and DNA ladder into wells on the gel.
- 5. Run the gel at roughly 100V for around an hour
Materials
- 10ml Luria Broth (LB)
- 10µl Specific Antibiotic at 1000x (Chloramphenicol, Ampicillin or Kanamycin)
- Loop (for picking colony)
- Ethanol
Procedure
- 1. Pour 10ml of LB into a 50ml Falcon tube.
- 2. Pipette 10µl of antibiotic into the broth.
- 3. Dip loop in ethanol and flame to sterilise. Once it is cool, pick colony and transfer to a 50ml Falcon tube.
- 4. Incubate at 37°C overnight in a shaking incubator.