Difference between revisions of "Team:UiOslo Norway/Experiments"

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<h1  style="font-size:400%;">Experiments</h1>
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<h2> 1. Obtaining the genes </h2>
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<p>The first project part pursues the goal to get or clone all genes that are involved in our project into a plasmid, which allows a rapid and easy amplification of those genes for further experiments. </br>
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For the soluble methane monooxygenase (sMMO) from <i>Methylococcus capsulatus</i> (Bath) we got the genes for the subunits (<i>mmoXYZBCD</i>) cloned into the BioBrick standard vector pSC1B3 from the
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<a href="https://2014.igem.org/Team:Braunschweig/Achievements-content#parts" >
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iGEM 2014 team from Braunschweig, Germany.
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Those six parts are registered as BioBrick parts under the names; Bba_K1390001 (<i>mmoB</i>), Bba_K1390002 (<i>mmoC</i>), Bba_K1390003 (<i>mmoD</i>), Bba_K1390004 (<i>mmoX</i>), Bba_K1390005 (<i>mmoY</i>), and Bba_K1390006 (<i>mmoZ</i>).
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<h1  style="font-size:400%;">Protocols</h1>
 
<h1  style="font-size:400%;">Protocols</h1>
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Revision as of 10:47, 1 September 2015

Experiments

1. Obtaining the genes

The first project part pursues the goal to get or clone all genes that are involved in our project into a plasmid, which allows a rapid and easy amplification of those genes for further experiments.
For the soluble methane monooxygenase (sMMO) from Methylococcus capsulatus (Bath) we got the genes for the subunits (mmoXYZBCD) cloned into the BioBrick standard vector pSC1B3 from the iGEM 2014 team from Braunschweig, Germany. Those six parts are registered as BioBrick parts under the names; Bba_K1390001 (mmoB), Bba_K1390002 (mmoC), Bba_K1390003 (mmoD), Bba_K1390004 (mmoX), Bba_K1390005 (mmoY), and Bba_K1390006 (mmoZ).

Protocols

Molecular biology

Protein Biochemistry

Handling bacteria