Difference between revisions of "Team:Freiburg/Parts"
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<div class="table sectionedit2"><table class="inline"> | <div class="table sectionedit2"><table class="inline"> | ||
<tr class="row0"> | <tr class="row0"> | ||
| − | <th class=" | + | <th class="col1 leftalign"> biobrick </th><th class="col1 leftalign"> short description </th><th class="col0 centeralign"> detailed desription </th> |
</tr> | </tr> | ||
<tr class="row1"> | <tr class="row1"> | ||
| − | + | <td class="col1"> <a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621000" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621000">BBa_K1621000</a></td><td class="col0"> Rubella Virus specific antigenic epitopes derived from glycoprotein E1</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_6" title="pRIG15_6">pRIG15_6</a></td> | |
</tr> | </tr> | ||
<tr class="row2"> | <tr class="row2"> | ||
| − | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621001" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621001">BBa_K1621001</a></td><td class="col0"> Varicella Zoster Virus specific antigenic epitopes derived from glycoprotein E</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_7" title="prig15_7">pRIG15_7</a></td> | |
</tr> | </tr> | ||
<tr class="row3"> | <tr class="row3"> | ||
| − | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621002" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621002">BBA_K1621002</a></td><td class="col0"> Herpes Simplex specific antigenic epitopes derived from glycoprotein G</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_8" title="prig15_8">pRIG15_8</a></td> | |
</tr> | </tr> | ||
<tr class="row4"> | <tr class="row4"> | ||
| − | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621003" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621003">BBa_K1621003</a></td><td class="col0"> <i> Clostridium tetani</i> specific antigenic epitopes derived from tetnus neurotoxin (TeNT_Hc)</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_11" title="prig15_11">pRIG15_11</a></td> | |
</tr> | </tr> | ||
<tr class="row5"> | <tr class="row5"> | ||
| − | <td class=" | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621004" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621004">BBa_K1621004</a></td><td class="col0"> Human Immunodeficiency Virus specific antigenic epitopes derived from a polyprotein called gag/tat/pol/env</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_17" title="prig15_17">pRIG15_17</a></td> |
</tr> | </tr> | ||
<tr class="row6"> | <tr class="row6"> | ||
| − | <td class=" | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621005" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621005">BBa_K1621005</a></td><td class="col0"> <i> Treponema pallidum</i> specific antigenic peptides derived from bacterioferritin (TpF1)</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_18" title="prig15_18">pRIG15_18</a></td> |
</tr> | </tr> | ||
<tr class="row7"> | <tr class="row7"> | ||
| − | <td class=" | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621006" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621006">BBa_K1621006</a></td><td class="col0"><i> Salmonella</i> Typhimurium specific antigenic protein (DHAD)<td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_15" title="prig15_15">pRIG15_15</a></td> |
</tr> | </tr> | ||
<tr class="row8"> | <tr class="row8"> | ||
| − | <td class=" | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621007" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621007">BBa_K1621007</a></td><td class="col0"> scFv binding specifically to the <i>Salmonella</i> Typhimurium derived antigen</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=prig15_13" title="prig15_13">pRIG15_13</a></td> |
</tr> | </tr> | ||
<tr class="row9"> | <tr class="row9"> | ||
| − | + | <td class="col1"><a class="urlextern" href="http://parts.igem.org/Part:BBa_K1621009" rel="nofollow" target="_Blank" title="http://parts.igem.org/Part:BBa_K1621009">BBa_K1621009</a></td><td class="col0"> Standardized plasmid backbone optimized for protein overexpression</td><td class="col0"><a class="wikilink1" href="/igem2015/doku.php?id=pop_-_protein_expression_meets_igem_standards" title="pop_-_protein_expression_meets_igem_standards">pOP</a></td> | |
</tr> | </tr> | ||
</table></div> | </table></div> | ||
Revision as of 09:47, 4 September 2015
Biobricks
Since our project involved expression of many antigens we decided to share those antigen-sequences with the iGEM community. To do this we used the codon optimization tool from Integrated DNA Technologies to improve most sequences for expression in E. coli. We also removed all restriction sites that are not allowed in RFC[10], so that all sequences are compatible with the submission vector pSB1C3. We obtained most of the sequences via paper research and we would like to give special thanks to the group of Prof. Dr. Michael Hust (TU Braunschweig) who provided us with expression plasmids for the Salmonella Typhimurium antigen and a corresponding single chain variable fragment. When we started working with our first plasmids for this project, we decided to use a specific nomenclature. Every plasmid name starts with “pIG15” which is short for “plasmid igem 2015”. According to this, we named the plasmids containing our biobricks in the shipping backbone pRIG15 (“pRIG” as in “brick”)
| biobrick | short description | detailed desription |
|---|---|---|
| BBa_K1621000 | Rubella Virus specific antigenic epitopes derived from glycoprotein E1 | pRIG15_6 |
| BBa_K1621001 | Varicella Zoster Virus specific antigenic epitopes derived from glycoprotein E | pRIG15_7 |
| BBA_K1621002 | Herpes Simplex specific antigenic epitopes derived from glycoprotein G | pRIG15_8 |
| BBa_K1621003 | Clostridium tetani specific antigenic epitopes derived from tetnus neurotoxin (TeNT_Hc) | pRIG15_11 |
| BBa_K1621004 | Human Immunodeficiency Virus specific antigenic epitopes derived from a polyprotein called gag/tat/pol/env | pRIG15_17 |
| BBa_K1621005 | Treponema pallidum specific antigenic peptides derived from bacterioferritin (TpF1) | pRIG15_18 |
| BBa_K1621006 | Salmonella Typhimurium specific antigenic protein (DHAD) | pRIG15_15 |
| BBa_K1621007 | scFv binding specifically to the Salmonella Typhimurium derived antigen | pRIG15_13 |
| BBa_K1621009 | Standardized plasmid backbone optimized for protein overexpression | pOP |
