Difference between revisions of "Team:Freiburg/Home"
m |
|||
Line 146: | Line 146: | ||
<div class="homepage_headline">How are we doing it?</div> | <div class="homepage_headline">How are we doing it?</div> | ||
<div id="how_text" class="homepage_text"> | <div id="how_text" class="homepage_text"> | ||
− | We | + | We produce protein arrays from a DNA-microarray template using cell-free expression. The DNA codes for antigens from many different pathogens. We then run blood serum over the protein array and measure whether antibodies bind to the antigens. We can observe that with a label-free optical measurement called iRIf. <a href="https://2015.igem.org/Team:Freiburg/Project/Overview"> Tell me more!</a> |
</div> | </div> | ||
</div> | </div> | ||
Line 158: | Line 158: | ||
<div id="why_text" class="homepage_text"> | <div id="why_text" class="homepage_text"> | ||
− | Protein arrays are a powerful way to screen for diseases, but they are very cumbersome to produce which makes them expensive. More | + | Protein arrays are a powerful way to screen for diseases, but they are very cumbersome to produce, which makes them quite expensive. More important, they denature quickly and have to be handled very carefully. Our DiaCHIP produces fresh protein arrays right before every measurement - and our DNA array allows us to xerox as many protein arrays as we want! <a href="https://2015.igem.org/Team:Freiburg/Project/Overview">Tell me more!</a> |
</div> | </div> | ||
</div> | </div> | ||
Line 179: | Line 179: | ||
</div> | </div> | ||
<div id="video_text" class="homepage_text"> | <div id="video_text" class="homepage_text"> | ||
− | Take a look at this short video clip, which will give you a general | + | Take a look at this short video clip, which will give you a general overview of what our project is about. |
</div> | </div> | ||
</div> | </div> |
Revision as of 11:09, 4 September 2015
![](https://static.igem.org/mediawiki/2015/8/85/Freiburg_wiki_background_2_parted_left.png)
![](https://static.igem.org/mediawiki/2015/f/f4/Freiburg_wiki_background_2_parted_right.png)
![](https://static.igem.org/mediawiki/2015/9/91/Freiburg_homepage_Dia_chip.png)
![](https://static.igem.org/mediawiki/2015/0/0f/Freiburg_homepage_chip_blood.png)
![](https://static.igem.org/mediawiki/2015/b/b6/Freiburg_homepage_chip_denature.png)
Abstract
In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference)which is based on a laser detecting the interference of reflecting light from our chip. Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.