Difference between revisions of "Team:NYU Shanghai/Parts"

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   <h3>Parts</h3>
 
   <h3>Parts</h3>
 
   <h4>We’ve created three different types of colored E. Coli.</h4>
 
   <h4>We’ve created three different types of colored E. Coli.</h4>
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   <img src="https://static.igem.org/mediawiki/2015/thumb/1/12/NYU_Shanghai_RFP.png/627px-NYU_Shanghai_RFP.png" width="250" height="250" style="margin:0px 5px">
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   <img src="https://static.igem.org/mediawiki/2015/thumb/a/a5/NYU_Shanghai_realchromoproteins.png/560px-NYU_Shanghai_realchromoproteins.png" width="250" height="250" style="margin:0px 5px">
 
   <p id="leftOverlay"><br><br><br><strong>Chromoproteins</strong><br>Pigmented compounds that absorb and reflect visible light.</p>
 
   <p id="leftOverlay"><br><br><br><strong>Chromoproteins</strong><br>Pigmented compounds that absorb and reflect visible light.</p>
 
   <img src="https://static.igem.org/mediawiki/2015/thumb/1/12/NYU_Shanghai_RFP.png/627px-NYU_Shanghai_RFP.png" width="250" height="250" style="margin:0px 5px">
 
   <img src="https://static.igem.org/mediawiki/2015/thumb/1/12/NYU_Shanghai_RFP.png/627px-NYU_Shanghai_RFP.png" width="250" height="250" style="margin:0px 5px">

Revision as of 23:39, 5 September 2015

Parts

We’ve created three different types of colored E. Coli.




Chromoproteins
Pigmented compounds that absorb and reflect visible light.




Fluorescent Proteins
Pigmented compounds that absorb UV light and emit visible light.




Luciferase Enzymes
Catalyze a reaction converting luciferin to oxyluciferin. Energy is released in the form of the light.

Chromoproteins

We created constructs to express 3 different colors of chromoproteins: blue, lime, and yellow. These were taken from the registry, created in 2010 by the Cambridge iGEM team.

BBa_K880005 promoter/rbs
BBa_K081005 promoter/rbs
Fluorescent Proteins

We utilized generators to express 2 different colors of fluorescent proteins: RFP and GFP. These parts from the registry.

BBa_J04450 RFP
BBa_J04451 RFP/rbs
Luciferase

We utilized generators to express 4 different bioluminescent colors: red, green, yellow, and orange.

BBa_K325219 Red Firefly Luciferase and LRE (under pBAD)
BBa_K325209 Green Firefly Luciferase and LRE (under pBAD)
Oscillating Luciferase

To create a natural beat generator, we created a light oscillating system. Unfortunately, the time scale was far too long for any practical beat generator (20min per beat). See Fudan 2012 Biowave for more information.