Difference between revisions of "Team:EPF Lausanne/Part Collection"
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<div class="col-md-6"> | <div class="col-md-6"> | ||
<h2>sgRNA Z0</h2> | <h2>sgRNA Z0</h2> | ||
| − | <p>the gRNA Z0 activates PAM rich URS J23117 promoter when in complex with dCas9.</p> | + | <p>the gRNA Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω.</p> |
| + | </div> | ||
| + | |||
| + | <div class="col-md-3 text-center"> | ||
| + | <br><br><br><br><br> | ||
| + | <a href="?"><b>BBa_TRUCMUCH</b></a> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="row"> | ||
| + | |||
| + | <div class="col-md-3 text-center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/5/50/EPF_Lausanne_Parts_Z4.png" style="width:75%"> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-md-6"> | ||
| + | <h2>sgRNA Z4</h2> | ||
| + | <p>the gRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω.</p> | ||
</div> | </div> | ||
Revision as of 16:23, 7 September 2015
dCas9-ω
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.
PAM rich URS J23117
This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.
sgRNA Z0
the gRNA Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω.
sgRNA Z4
the gRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω.
