Difference between revisions of "Team:EPF Lausanne/Part Collection"
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<div class="col-md-6"> | <div class="col-md-6"> | ||
<h2>sgRNA Z0</h2> | <h2>sgRNA Z0</h2> | ||
| − | <p>the | + | <p>the sgRNA (single guide-RNA) Z0 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z0 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p> |
</div> | </div> | ||
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<div class="col-md-6"> | <div class="col-md-6"> | ||
<h2>sgRNA Z4</h2> | <h2>sgRNA Z4</h2> | ||
| − | <p>the | + | <p>the sgRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z4 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p> |
</div> | </div> | ||
| Line 102: | Line 102: | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
<h2>sgRNA Z35</h2> | <h2>sgRNA Z35</h2> | ||
| − | <p>the | + | <p>the sgRNA Z35 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z35 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.</p> |
| + | </div> | ||
| + | |||
| + | <div class="col-md-3 text-center"> | ||
| + | <br><br><br><br><br> | ||
| + | <a href="?"><b>BBa_TRUCMUCH</b></a> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="row"> | ||
| + | <div class="col-md-3 text-center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/1/17/EPF_Lausanne_Parts_J23117Alt.png" style="width:75%"> | ||
| + | </div> | ||
| + | |||
| + | <div class="col-md-6"> | ||
| + | <h2>PAM rich URS J23117Alt</h2> | ||
| + | <p>This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to obtain different sequences for sgRNA targeting. It is also fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind, as dCas9 cannot bind without a PAM sequence.</p> | ||
</div> | </div> | ||
Revision as of 17:25, 7 September 2015
E. coli.
dCas9-ω
dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.
PAM rich URS J23117
This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.
sgRNA Z0
the sgRNA (single guide-RNA) Z0 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z0 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.
sgRNA Z4
the sgRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z4 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.
sgRNA Z35
the sgRNA Z35 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z35 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.
PAM rich URS J23117Alt
This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to obtain different sequences for sgRNA targeting. It is also fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind, as dCas9 cannot bind without a PAM sequence.
