Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

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<p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:</p>
 
<p>Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:</p>
 
<div align=center>
 
<div align=center>
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<font color="017e70"><b>REPLACE LIST WITH TABLE AND/OR FIGURE</b></font>
 
BBa_K864100+BBa_K314100<p>
 
BBa_K864100+BBa_K314100<p>
 
BBa_E0020+BBa_K608007</p><p>
 
BBa_E0020+BBa_K608007</p><p>
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<font face="Courier New">Fluorescence Readings</font>
 
<font face="Courier New">Fluorescence Readings</font>
 
<hr>
 
<hr>
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[[Image:2015_Austin_UTexas_SpringsemesterBB-summary.png‎|500px|thumb|right|<b>Fig.2</b> description here]]
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[[Image:2015_Austin_UTexas_SpringsemesterBB-summary.png‎|500px|thumb|right|<b>Fig.2</b> ^^^REPLACE WITH BETTER RESOLUTION]]
 
<font color="017e70">relative fluorescence units<br>
 
<font color="017e70">relative fluorescence units<br>
 
96 well plate<br>
 
96 well plate<br>
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<br>
 
<br>
 
<br>
 
<br>
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<font face="Courier New">Protocol</font>
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<hr>
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<br><br>
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<b><font color="017e70">(WILL BE MOVED TO FIRST DATA SET)</font></b>
 
<font face="Courier New">Observations and Discussion</font>
 
<font face="Courier New">Observations and Discussion</font>
 
<hr>
 
<hr>

Revision as of 23:10, 10 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:

REPLACE LIST WITH TABLE AND/OR FIGURE

BBa_K864100+BBa_K314100

BBa_E0020+BBa_K608007

BBa_EE030+BBa_K608006

BBa_E0020+BBa_K608003

BBa_E0030+BBa_K608007

BBa_K592101+BBa_K608006

BBa_K592100+BBa_K608004

BBa_K592101+BBa_K608002

BBa_K864100+BBa_K608006

BBa_E0030+BBa_K314100

BBa_E0020+BBa_K608002

BBa_K864100+BBa_K608002

BBa_E0030+BBa_K314100

BBa_K592101+BBa_K608007


discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Protocol



(WILL BE MOVED TO FIRST DATA SET) Observations and Discussion

insert mutations in SFYFP
EYFP, CYFP stable
what contributes to stability
what does this mean about what sequences are stable</br>
next steps