Difference between revisions of "Team:NYU Shanghai/Protocols"
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Estimated time: 3 hours (plus 14-18 hour incubation) | Estimated time: 3 hours (plus 14-18 hour incubation) | ||
<br>Materials | <br>Materials | ||
− | <ul> | + | <ul style="display:none"> |
<li>2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes)</li> | <li>2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes)</li> | ||
<li>Competent cells (50ul per transformation)</li> | <li>Competent cells (50ul per transformation)</li> | ||
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</ul> | </ul> | ||
<br><br>Procedure | <br><br>Procedure | ||
− | <ol> | + | <ol style="display:none"> |
<li>Start thawing the competent cells on ice.</li> | <li>Start thawing the competent cells on ice.</li> | ||
<li>Add 50 µL of thawed competent cells into pre-chilled 2ml tube, and another 50µL into a 2ml tube, labelled for your control.</li> | <li>Add 50 µL of thawed competent cells into pre-chilled 2ml tube, and another 50µL into a 2ml tube, labelled for your control.</li> |
Revision as of 19:05, 11 September 2015