Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

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<font face="Courier New">Protocol</font>
 
<font face="Courier New">Protocol</font>
 
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<b><font color="017e70">(WILL BE MOVED TO FIRST DATA SET)</font></b>
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<font face="Courier New">Observations and Discussion</font>
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<font color="017e70">insert mutations in SFYFP<br>
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EYFP, CYFP stable<br>
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what contributes to stability<br>
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what does this mean about what sequences are stable</br>
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next steps<br>
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Revision as of 19:14, 11 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:

REPLACE LIST WITH TABLE AND/OR FIGURE

BBa_K864100+BBa_K314100

BBa_E0020+BBa_K608007

BBa_EE030+BBa_K608006

BBa_E0020+BBa_K608003

BBa_E0030+BBa_K608007

BBa_K592101+BBa_K608006

BBa_K592100+BBa_K608004

BBa_K592101+BBa_K608002

BBa_K864100+BBa_K608006

BBa_E0030+BBa_K314100

BBa_E0020+BBa_K608002

BBa_K864100+BBa_K608002

BBa_E0030+BBa_K314100

BBa_K592101+BBa_K608007


discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Protocol