Difference between revisions of "Team:NYU Shanghai/Protocols"
m |
m |
||
Line 225: | Line 225: | ||
Estimated time: 3 hours (plus 14-18 hour incubation) | Estimated time: 3 hours (plus 14-18 hour incubation) | ||
<br><br>Materials | <br><br>Materials | ||
− | <li | + | <li>2µl resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes) |
− | <li | + | <li>Competent cells (50ul per transformation)</li> |
− | <li | + | <li>Ice (in ice bucket/container)</li> |
− | <li | + | <li>2ml tube (1 per a transformation')</li> |
− | <li | + | <li>42ºC water bath</li> |
− | <li | + | <li>SOC media</li> |
− | <li | + | <li>1 small LB plate and 1 large LB+antibiotic per transformation</li> |
− | <li | + | <li>Spreader</li> |
− | <li | + | <li>37ºC incubator</li> |
<br><br>Procedure | <br><br>Procedure | ||
− | <ol | + | <ol> |
<li>Start thawing the competent cells on ice.</li> | <li>Start thawing the competent cells on ice.</li> | ||
<li>Add 50 µL of thawed competent cells into pre-chilled 2ml tube, and another 50µL into a 2ml tube, labelled for your control.</li> | <li>Add 50 µL of thawed competent cells into pre-chilled 2ml tube, and another 50µL into a 2ml tube, labelled for your control.</li> |
Revision as of 19:15, 11 September 2015