Difference between revisions of "Team:UMaryland/Description"
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<br> | <br> | ||
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| + | <p style="font-size:64px"><b>Safe and Inexpensive</b></style> | ||
<br> | <br> | ||
| − | <p style="font-size: | + | <p style="font-size:64px"><u><b>Approaches to Advance Synthetic Biology</b></u></style> |
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| − | <a href=" | + | <a href="https://2015.igem.org/Team:UMaryland/Interlab"> |
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<h4>IL Study</h4> | <h4>IL Study</h4> | ||
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| − | <a href=" | + | <a href="https://2015.igem.org/Team:UMaryland/sideprojects"> |
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<h4>Side projects</h4> | <h4>Side projects</h4> | ||
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<p style="font-size:18px">How does this lead to plasmid maintenance? Hok mRNA, due to a high degree of secondary structure, has a long half-life, measured at 20 minutes. Sok, on the other hand, has a half-life of only 30 seconds. The cell must thus retain the coding region for Sok in order to produce enough Sok to continue blocking Hok translation. If the plasmid is lost, then both the Hok and Sok coding regions will be lost; however, previously transcribed Hok mRNA will still be present. With previously transcribed Sok rapidly degrading, Hok mRNA will be translated, killing the daughter cell that did not maintain the plasmid.</p> | <p style="font-size:18px">How does this lead to plasmid maintenance? Hok mRNA, due to a high degree of secondary structure, has a long half-life, measured at 20 minutes. Sok, on the other hand, has a half-life of only 30 seconds. The cell must thus retain the coding region for Sok in order to produce enough Sok to continue blocking Hok translation. If the plasmid is lost, then both the Hok and Sok coding regions will be lost; however, previously transcribed Hok mRNA will still be present. With previously transcribed Sok rapidly degrading, Hok mRNA will be translated, killing the daughter cell that did not maintain the plasmid.</p> | ||
| − | < | + | <a href="https://2015.igem.org/Team:UMaryland/Design"> |
| + | <div id='button2'> | ||
| + | <p>Click here to learn more about our design | ||
| + | </div> | ||
| + | </a> | ||
</div> | </div> | ||
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<div id='layer3'> | <div id='layer3'> | ||
<div id='contentbox'> | <div id='contentbox'> | ||
| − | <a name="PCR"><h1>Thermocycler</h1></a> | + | <a name="PCR"><h1><b>Thermocycler</b></h1></a> |
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<p style="font-size:18px"> | <p style="font-size:18px"> | ||
| − | PCR is often a common tool in the laboratory environment. It is also a vital component in DNA amplification and any cloning procedure. However the majority of theses devices are bulky expensive machines that are rarely seen outside of bio based labs. This was the premise for the development of CHIP or Cheap Homemade Innovative PCR, development of a compact PCR machine that was as fast and proficient at amplifying DNA as lab based machines but for the fraction of the price and for teaching based applications. We are proud to say that we have been able to design a machine that will be completely open source and able to serve as a platform for DIY cloning procedures. | + | PCR is often a common tool in the laboratory environment. It is also a vital component in DNA amplification and any cloning procedure. However the majority of theses devices are bulky expensive machines that are rarely seen outside of bio based labs. This was the premise for the development of CHIP or Cheap Homemade Innovative PCR, development of a compact PCR machine that was as fast and proficient at amplifying DNA as lab based machines but for the fraction of the price and for teaching based applications. We are proud to say that we have been able to design a machine that will be completely open source and able to serve as a platform for DIY cloning procedures. |
| − | + | <a href="https://2015.igem.org/Team:UMaryland/Hardware"> | |
| − | < | + | <div id='button2'> |
| + | <p>Click here to learn more about our PCR | ||
| + | </div> | ||
| + | </a> | ||
</div> | </div> | ||
Revision as of 20:56, 11 September 2015
