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Revision as of 12:53, 13 September 2015

""

Julia: Bin grad dabei das hier komplett neu zu machen, nach Plan vom Website-Team.

Diagnostics

The following section summarizes the most interesting results we obtained this summer establishing our diagnostic tool. On our way to the detection of anti-Tetanus in human blood serum (link tetanus) we achieved many other results in the field of Diagnosis. Besides the detection of anti-Tetanus antibodies we could identify anti-GFP antibodies in a more or less 20 years old serum of a rabbit that was immunized with GFP. (link main results). Before we achieved these main results we proofed our concept, showing that our device is indeed capable of detecting specific antigen-antibody binding.

Detection of anti-Salmonella

We obtained the sequence for a specific Salmonella Typhimurium antigen and a corresponding antibody from the lab of Prof. Dr. Hust. We successfully expressed both proteins in E. coli and spotted the His-tagged antigen on a specific PDITC surface. With the following iRIf measurement we analyzed the binding between antigen and antibody using our own DiaCHIP. The measurement was a great success, showing a distinct shift in the binding curve for the salmonella antigen when the salmonella antibody is flushed over the chip, whereas the negative control is not bound by the antibody. With this measurement a proof of concept can be provided, showing that our device is indeed capable of detecting specific antigen-antibody binding, as it will be occurring when patient samples are analyzed.

Figure 1: Binding of anti-Salmonella

Mouse and rabbit binding

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expression of several antigens

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