Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

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     <td><b><u>Insert BioBrick</u></b></td>
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     <td><b><u>Fluorescent Protein BioBrick</u></b></td>
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     <td><b><u>Insert Phenotype</u></b></td>
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     <td><b><u>Fluorescent Protein</u></b></td>
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     <td><b><u>Vector BioBrick</u></b></td>
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     <td><b><u>Promoter/RBS BioBrick</u></b></td>
 
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   </tr>
 
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Revision as of 21:52, 13 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:


Fluorescent Protein BioBrick Fluorescent Protein Promoter/RBS BioBrick
BBa_K592100 BFP BBa_K608004
BBa_E0020 ECFP BBa_K608007
BBa_E0020 ECFP BBa_K608003
BBa_E0020 ECFP BBa_K608002
BBa_K592101 YFP BBa_K608002
BBa_K592101 YFP BBa_K608006
BBa_K592101 YFP BBa_K608007
BBa_K864100 SYFP BBa_K608002
BBa_K864100 SYFP BBa_K314100
BBa_864100 SYFP BBa_K608006
BBa_E0030 EYFP BBa_K608007
BBa_EE030 EYFP BBa_K608006
BBa_E0030 EYFP BBa_K314100
BBa_E0030 EYFP BBa_K314100


discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Protocol