Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

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<tr>
 
<tr>
 
     <td>BBa_K864100</td>
 
     <td>BBa_K864100</td>
     <td>SYFP</td>
+
     <td>SYFP2</td>
 
     <td>BBa_K608002</td>
 
     <td>BBa_K608002</td>
 
   </tr>
 
   </tr>
 
  <tr>
 
  <tr>
 
     <td>BBa_K864100</td>
 
     <td>BBa_K864100</td>
     <td>SYFP</td>
+
     <td>SYFP2</td>
 
     <td>BBa_K314100</td>
 
     <td>BBa_K314100</td>
 
   </tr>
 
   </tr>
 
<tr>
 
<tr>
     <td>BBa_864100</td>
+
     <td>BBa_K864100</td>
     <td>SYFP</td>
+
     <td>SYFP2</td>
 
     <td>BBa_K608006</td>
 
     <td>BBa_K608006</td>
 
   </tr>
 
   </tr>

Revision as of 21:55, 13 September 2015

--this is a first draft--

Spring 2015 Data Set



Plasmid Construction and Transformation



Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:



Fluorescent Protein BioBrick Fluorescent Protein Promoter/RBS BioBrick
BBa_K592100 BFP BBa_K608004
BBa_E0020 ECFP BBa_K608007
BBa_E0020 ECFP BBa_K608003
BBa_E0020 ECFP BBa_K608002
BBa_K592101 YFP BBa_K608002
BBa_K592101 YFP BBa_K608006
BBa_K592101 YFP BBa_K608007
BBa_K864100 SYFP2 BBa_K608002
BBa_K864100 SYFP2 BBa_K314100
BBa_K864100 SYFP2 BBa_K608006
BBa_E0030 EYFP BBa_K608007
BBa_EE030 EYFP BBa_K608006
BBa_E0030 EYFP BBa_K314100
BBa_E0030 EYFP BBa_K314100


discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings


Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)


Protocol