Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

m
Line 26: Line 26:
 
     <td>BFP</td>
 
     <td>BFP</td>
 
     <td>BBa_K608004</td>
 
     <td>BBa_K608004</td>
 +
  </tr>
 +
<tr>
 +
    <td>BBa_K592100</td>
 +
    <td>BFP</td>
 +
    <td>BBa_K608007</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>

Revision as of 21:56, 13 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:


Fluorescent Protein BioBrick Fluorescent Protein Promoter/RBS BioBrick
BBa_K592100 BFP BBa_K608004
BBa_K592100 BFP BBa_K608007
BBa_E0020 ECFP BBa_K608007
BBa_E0020 ECFP BBa_K608003
BBa_E0020 ECFP BBa_K608002
BBa_K592101 YFP BBa_K608002
BBa_K592101 YFP BBa_K608006
BBa_K592101 YFP BBa_K608007
BBa_K864100 SYFP2 BBa_K608002
BBa_K864100 SYFP2 BBa_K314100
BBa_K864100 SYFP2 BBa_K608006
BBa_E0030 EYFP BBa_K608007
BBa_EE030 EYFP BBa_K608006
BBa_E0030 EYFP BBa_K314100
BBa_E0030 EYFP BBa_K314100


discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Protocol