Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"
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| − | <table style="width: | + | <table style="width:90%"> |
<tr> | <tr> | ||
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<td><b><u>Fluorescent Protein</u></b></td> | <td><b><u>Fluorescent Protein</u></b></td> | ||
<td><b><u>Promoter/RBS BioBrick</u></b></td> | <td><b><u>Promoter/RBS BioBrick</u></b></td> | ||
| + | <td><b><u>Promoter;RBS Strength</u></b></td> | ||
</tr> | </tr> | ||
<tr></tr> | <tr></tr> | ||
| Line 26: | Line 27: | ||
<td>BFP</td> | <td>BFP</td> | ||
<td>BBa_K608004</td> | <td>BBa_K608004</td> | ||
| + | <td>Strong; Weak</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 31: | Line 33: | ||
<td>BFP</td> | <td>BFP</td> | ||
<td>BBa_K608007</td> | <td>BBa_K608007</td> | ||
| + | <td>Medium; Weak</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 36: | Line 39: | ||
<td>ECFP</td> | <td>ECFP</td> | ||
<td>BBa_K608007</td> | <td>BBa_K608007</td> | ||
| + | <td>Medium; Weak</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 41: | Line 45: | ||
<td>ECFP</td> | <td>ECFP</td> | ||
<td>BBa_K608003</td> | <td>BBa_K608003</td> | ||
| + | <td>Strong; Medium</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 46: | Line 51: | ||
<td>ECFP</td> | <td>ECFP</td> | ||
<td>BBa_K608002</td> | <td>BBa_K608002</td> | ||
| + | <td>Strong; Strong</td> | ||
</tr> | </tr> | ||
<hr> | <hr> | ||
| Line 52: | Line 58: | ||
<td>YFP</td> | <td>YFP</td> | ||
<td>BBa_K608002</td> | <td>BBa_K608002</td> | ||
| + | <td>Strong; Strong</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 57: | Line 64: | ||
<td>YFP</td> | <td>YFP</td> | ||
<td>BBa_K608006</td> | <td>BBa_K608006</td> | ||
| − | + | <td>Medium; Medium</td> | |
| + | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K592101</td> | <td>BBa_K592101</td> | ||
<td>YFP</td> | <td>YFP</td> | ||
<td>BBa_K608007</td> | <td>BBa_K608007</td> | ||
| + | <td>Medium; Weak</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 67: | Line 76: | ||
<td>SYFP2</td> | <td>SYFP2</td> | ||
<td>BBa_K608002</td> | <td>BBa_K608002</td> | ||
| + | <td>Strong; Strong</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 72: | Line 82: | ||
<td>SYFP2</td> | <td>SYFP2</td> | ||
<td>BBa_K314100</td> | <td>BBa_K314100</td> | ||
| − | + | <td>Strong; Very Strong</td> | |
| + | </tr> | ||
<tr> | <tr> | ||
<td>BBa_K864100</td> | <td>BBa_K864100</td> | ||
<td>SYFP2</td> | <td>SYFP2</td> | ||
<td>BBa_K608006</td> | <td>BBa_K608006</td> | ||
| + | <td>Medium; Medium</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 82: | Line 94: | ||
<td>EYFP</td> | <td>EYFP</td> | ||
<td>BBa_K608007</td> | <td>BBa_K608007</td> | ||
| + | <td>Medium; Weak</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 87: | Line 100: | ||
<td>EYFP</td> | <td>EYFP</td> | ||
<td>BBa_K608006</td> | <td>BBa_K608006</td> | ||
| − | + | <td>Medium; Medium</td> | |
| − | + | </tr> | |
| − | + | ||
| − | + | ||
| − | <td> | + | |
| − | + | ||
<tr> | <tr> | ||
<td>BBa_E0030</td> | <td>BBa_E0030</td> | ||
<td>EYFP</td> | <td>EYFP</td> | ||
<td>BBa_K314100</td> | <td>BBa_K314100</td> | ||
| + | <td>Strong; Very Strong</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 22:43, 13 September 2015
--this is a first draft--
Spring 2015 Data Set
Plasmid Construction and Transformation
Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:
| Fluorescent Protein BioBrick | Fluorescent Protein | Promoter/RBS BioBrick | Promoter;RBS Strength |
| BBa_K592100 | BFP | BBa_K608004 | Strong; Weak |
| BBa_K592100 | BFP | BBa_K608007 | Medium; Weak |
| BBa_E0020 | ECFP | BBa_K608007 | Medium; Weak |
| BBa_E0020 | ECFP | BBa_K608003 | Strong; Medium |
| BBa_E0020 | ECFP | BBa_K608002 | Strong; Strong |
| BBa_K592101 | YFP | BBa_K608002 | Strong; Strong |
| BBa_K592101 | YFP | BBa_K608006 | Medium; Medium |
| BBa_K592101 | YFP | BBa_K608007 | Medium; Weak |
| BBa_K864100 | SYFP2 | BBa_K608002 | Strong; Strong |
| BBa_K864100 | SYFP2 | BBa_K314100 | Strong; Very Strong |
| BBa_K864100 | SYFP2 | BBa_K608006 | Medium; Medium |
| BBa_E0030 | EYFP | BBa_K608007 | Medium; Weak |
| BBa_EE030 | EYFP | BBa_K608006 | Medium; Medium |
| BBa_E0030 | EYFP | BBa_K314100 | Strong; Very Strong |
