Difference between revisions of "Team:Austin UTexas/Project/Plasmid Study"

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<font color="017e70">discuss what these BioBricks are<br>
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<font color="017e70">plasmids transformed by electroporation into pSB1c3</font>
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Revision as of 22:44, 13 September 2015

--this is a first draft--

Spring 2015 Data Set

Plasmid Construction and Transformation


Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:


Fluorescent Protein BioBrick Fluorescent Protein Promoter/RBS BioBrick Promoter;RBS Strength
BBa_K592100 BFP BBa_K608004 Strong; Weak
BBa_K592100 BFP BBa_K608007 Medium; Weak
BBa_E0020 ECFP BBa_K608007 Medium; Weak
BBa_E0020 ECFP BBa_K608003 Strong; Medium
BBa_E0020 ECFP BBa_K608002 Strong; Strong
BBa_K592101 YFP BBa_K608002 Strong; Strong
BBa_K592101 YFP BBa_K608006 Medium; Medium
BBa_K592101 YFP BBa_K608007 Medium; Weak
BBa_K864100 SYFP2 BBa_K608002 Strong; Strong
BBa_K864100 SYFP2 BBa_K314100 Strong; Very Strong
BBa_K864100 SYFP2 BBa_K608006 Medium; Medium
BBa_E0030 EYFP BBa_K608007 Medium; Weak
BBa_EE030 EYFP BBa_K608006 Medium; Medium
BBa_E0030 EYFP BBa_K314100 Strong; Very Strong


plasmids transformed by electroporation into pSB1c3



Fluorescence Readings

Fig.2 ^^^REPLACE WITH BETTER RESOLUTION

relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)

Protocol