Difference between revisions of "Team:NYU Shanghai/Protocols"
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<br> | <br> | ||
<div id="SOCText" style="display:none"> | <div id="SOCText" style="display:none"> | ||
− | This is | + | Materials |
+ | <ul> | ||
+ | <li>0.5% (w/v) yeast extract</li> | ||
+ | <li>2% (w/v) tryptone</li> | ||
+ | <li>10 mM NaCl</li> | ||
+ | <li>2.5 mM KCl</li> | ||
+ | <li>20 mM MgSO4</li> | ||
+ | </ul><br><br>Per liter:<ul> | ||
+ | <li>5 g yeast extract</li> | ||
+ | <li>20 g tryptone</li> | ||
+ | <li>0.584 g NaCl</li> | ||
+ | <li>0.186 g KCl</li> | ||
+ | <li>2.4 g MgSO4</li> | ||
+ | </ul><br><br> | ||
+ | <p><em>Note:</em> Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4. | ||
+ | SOB medium is also available dry premixed from Difco, 0443-17.<br>Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.<br><b>15/10 medium</b><br>Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:</p> | ||
+ | <ul> | ||
+ | <li>1.5% yeast extract</li> | ||
+ | <li>1% Bacto-Tryptone</li> | ||
+ | <li>10mM NaCl</li> | ||
+ | <li>2mM KCl</li> | ||
+ | <li>10 mM MgCl2</li> | ||
+ | <li>10 mM MgSO4</li> | ||
+ | </ul> | ||
+ | |||
</div> | </div> | ||
</div> | </div> |
Revision as of 05:48, 14 September 2015