Difference between revisions of "Team:Freiburg/Protocols/MesurementGFP"
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− | + | <li class="level1"><div class="li">All fluorescence measurements were performed with a microplate reader in blach plates with transparent bottom </div> | |
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− | <li class="level1"><div class="li"> | + | <li class="level1"><div class="li">As Exitation wavelength 480nm were choosen </div> |
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+ | <li class="level1"><div class="li">Emission was measured at 520nm or a full spectrum was taken from 500 to 700 nm</div> | ||
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+ | <li class="level1"><div class="li">Reactions were either performed in a tube and transferred to the microplate after the cell-free reaction or directly performed inside the platereader </div> | ||
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+ | <li class="level1"><div class="li">when the reaction was performed inside the Platreader the wells were covered with foil to avoid evaporation</div> | ||
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+ | <li class="level1"><div class="li"> After the reaction and Measurement samples were stored at 4°C for further folding or directly frozen at -20°C</div> | ||
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Revision as of 22:56, 14 September 2015
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Measurement of GFP Expression
Measurement of expressed GFP
- All fluorescence measurements were performed with a microplate reader in blach plates with transparent bottom
- As Exitation wavelength 480nm were choosen
- Emission was measured at 520nm or a full spectrum was taken from 500 to 700 nm
- Reactions were either performed in a tube and transferred to the microplate after the cell-free reaction or directly performed inside the platereader
- when the reaction was performed inside the Platreader the wells were covered with foil to avoid evaporation
- After the reaction and Measurement samples were stored at 4°C for further folding or directly frozen at -20°C